Journal article
Control of ethanol sensitivity of the glycine receptor α3 subunit by transmembrane 2, the intracellular splice cassette and C-terminal domains
Journal of Pharmacology and Experimental Therapeutics, Vol.353(1), pp.80-90
2015
Abstract
Previous studies have shown that the efrfect of ethanol on glycine receptors (GlyRs) containing the α1 subunit is affected by interaction with heterotrimeric G proteins (Gbg). GlyRs containing the α3 subunit are involved in inflammatory pain sensitization and rhythmic breathing and have received much recent attention. For example, it is unknown whether ethanol affects the function of this important GlyR subtype. Electrophysiologic experiments showed that GlyR α3 subunits were not potentiated by pharmacologic concentrations of ethanol or by Gbg. Thus, we studied GlyR α1-α3 chimeras and mutants to determine the molecular properties that confer ethanol insensitivity. Mutation of corresponding glycine 254 in transmembrane domain 2 (TM2) found in α1 in the α3A254G-α1 chimera induced a glycine-evoked current that displayed potentiation during application of ethanol (46±5%, 100 mM) and Gβγ activation (80±17%). Interestingly, insertion of the intracellular α3L splice cassette into GlyR α1 abolished the enhancement of the glycine-activated current by ethanol (5±6%) and activation by G7βγ (21±7%). Incorporation of the GlyR α1 C terminus into the ethanol-resistant α3SA254G mutant produced a construct that displayed potentiation of the glycine-activated current with 100 mM ethanol (40±6%) together with a current enhancement after G protein activation (68±25%). Taken together, these data demonstrate that GlyR α3 subunits are not modulated by ethanol. Residue A254 in TM2, the α3L splice cassette, and the C-terminal domain of α3 GlyRs are determinants for low ethanol sensitivity and form the molecular basis of subtype-selective modulation of GlyRs by alcohol. Copyright © 2015 by The American Society for Pharmacology and Experimental Therapeutics.
Details
- Title
- Control of ethanol sensitivity of the glycine receptor α3 subunit by transmembrane 2, the intracellular splice cassette and C-terminal domains
- Authors
- A Sánchez (Author) - University of Concepción, ChileG E Yévenes (Author) - University of Concepción, ChileL S Martin (Author) - University of Concepción, ChileC F Burgos (Author) - University of Concepción, ChileG Moraga-Cid (Author) - University of Concepción, ChileRobert J Harvey (Author) - University college London, United KingdomL G Aguayo (Author) - University of Concepción, Chile
- Publication details
- Journal of Pharmacology and Experimental Therapeutics, Vol.353(1), pp.80-90
- Publisher
- American Society for Pharmacology and Experimental Therapeutics
- Date published
- 2015
- DOI
- 10.1124/jpet.114.221143
- ISSN
- 1521-0103
- Organisation Unit
- School of Health; University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; Centre for Bioinnovation; School of Health and Behavioural Sciences - Legacy
- Language
- English
- Record Identifier
- 99451207102621
- Output Type
- Journal article
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