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Complete Nucleotide Sequence of the Chloroplast Genome from the Tasmanian Blue Gum, Eucalyptus globulus (Myrtaceae)
Journal article   Peer reviewed

Complete Nucleotide Sequence of the Chloroplast Genome from the Tasmanian Blue Gum, Eucalyptus globulus (Myrtaceae)

Dorothy A Steane
DNA Research, Vol.12(3), pp.215-220
2005
url
https://doi.org/10.1093/dnares/dsi006View
Published Version

Abstract

Genetics eucalypt myrtaceae chloroplast DNA pseudogene gymnosperm
The complete nucleotide sequence of the chloroplast genome of the hardwood species Eucalyptus globulus ispresented and compared with chloroplast genomes of tree and non-tree angiosperms and two softwood treespecies. The 160 286 bp genome is similar in gene order to that of Nicotiana, with an inverted repeat (IR)(26 393 bp) separated by a large single copy (LSC) region of 89 012 bp and a small single copy region of18 488 bp. There are 128 genes(112 individual gene speciesand 16 genesdupli cated in the inverted repeat)coding for 30 transfer RNAs, 4 ribosomal RNAs and 78 proteins. One pseudogene (c-infA) and one pseudo-ycf(c-ycf15) were identified. The chloroplast genome of E. globulus is essentially co-linear with that of anotherhardwood tree species, Populus trichocarpa, except that the latter lacks rps16 and rpl32, and the IR hasexpanded in Populus to include rps19 (part of the LSC in E. globulus). Since the chloroplast genome ofE. globulus is not significantly different from other tree and non-tree angiosperm taxa, a comparison ofhardwood and softwood chloroplasts becomes, in essence, a comparison of angiosperm and gymnospermchloroplasts. When compared with E. globulus, Pinus chloroplasts have a very small IR, two extra tRNAs andfour additional photosynthetic genes, lack any functional ndh genesand have a significantly different genomearrangement. There doesnot appear to be any correlation between plant habit and chloroplast genomecomposition and arrangement.

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