Complementary Imaging of Silver Nanoparticle Interactions with Green Algae: Dark-Field Microscopy, Electron Microscopy, and Nanoscale Secondary Ion Mass Spectrometry

Ryo Sekine; K L Moore; M Matzke; P Vallotton; H Jiang; G M Hughes; J K Kirby; E Donner; C R M Grovenor; C Svendsen; E Lombi

doi:10.1021/acsnano.7b04556

Back

Complementary Imaging of Silver Nanoparticle Interactions with Green Algae: Dark-Field Microscopy, Electron Microscopy, and Nanoscale Secondary Ion Mass Spectrometry

Journal article

Open access

Peer reviewed

Complementary Imaging of Silver Nanoparticle Interactions with Green Algae: Dark-Field Microscopy, Electron Microscopy, and Nanoscale Secondary Ion Mass Spectrometry

Ryo Sekine, K L Moore, M Matzke, P Vallotton, H Jiang, G M Hughes, J K Kirby, E Donner, C R M Grovenor, C Svendsen, …

ACS Nano, Vol.11(11), pp.10894-10902

2017

DOI: https://doi.org/10.1021/acsnano.7b04556

Files and links (2)

pdf

PDF - Published Version (Open Access)4.31 MBDownload View

Published VersionPDF - Published Version (Open Access)CC BY V4.0, Open Access

url

https://doi.org/10.1021/acsnano.7b04556View

Published Version

Abstract

bio-nano interactions

dark-field light microscopy

multimodal imaging

NanoSIMS

nanotoxicology

silver nanoparticles

Increasing consumer use of engineered nanomaterials has led to significantly increased efforts to understand their potential impact on the environment and living organisms. Currently, no individual technique can provide all the necessary information such as their size, distribution, and chemistry in complex biological systems. Consequently, there is a need to develop complementary instrumental imaging approaches that provide enhanced understanding of these "bio-nano" interactions to overcome the limitations of individual techniques. Here we used a multimodal imaging approach incorporating dark-field light microscopy, high-resolution electron microscopy, and nanoscale secondary ion mass spectrometry (NanoSIMS). The aim was to gain insight into the bio-nano interactions of surface-functionalized silver nanoparticles (Ag-NPs) with the green algae Raphidocelis subcapitata, by combining the fidelity, spatial resolution, and elemental identification offered by the three techniques, respectively. Each technique revealed that Ag-NPs interact with the green algae with a dependence on the size (10 nm vs 60 nm) and surface functionality (tannic acid vs branched polyethylenimine, bPEI) of the NPs. Dark-field light microscopy revealed the presence of strong light scatterers on the algal cell surface, and SEM imaging confirmed their nanoparticulate nature and localization at nanoscale resolution. NanoSIMS imaging confirmed their chemical identity as Ag, with the majority of signal concentrated at the cell surface. Furthermore, SEM and NanoSIMS provided evidence of 10 nm bPEI Ag-NP internalization at higher concentrations (40 μg/L), correlating with the highest toxicity observed from these NPs. This multimodal approach thus demonstrated an effective approach to complement dose-response studies in nano-(eco)-toxicological investigations.

Details

Title: Complementary Imaging of Silver Nanoparticle Interactions with Green Algae: Dark-Field Microscopy, Electron Microscopy, and Nanoscale Secondary Ion Mass Spectrometry
Authors: Ryo Sekine (Author) - University of South Australia
K L Moore (Author) - University of Oxford, United Kingdom
M Matzke (Author) - Centre for Ecology and Hydrology, United Kingdom
P Vallotton (Author) - ETH Zurich, Switzerland
H Jiang (Author) - University of Oxford, United Kingdom
G M Hughes (Author) - University of Oxford, United Kingdom
J K Kirby (Author) - CSIRO Land and Water
E Donner (Author) - University of South Australia
C R M Grovenor (Author) - University of Oxford, United Kingdom
C Svendsen (Author) - Centre for Ecology and Hydrology, United Kingdom
E Lombi (Author) - University of South Australia
Publication details: ACS Nano, Vol.11(11), pp.10894-10902
Publisher: American Chemical Society
Date published: 2017
DOI: 10.1021/acsnano.7b04556
ISSN: 1936-0851
Copyright note: Copyright © 2017 American Chemical Society. This is an open access article published under a Creative Commons Attribution (CC-BY) License, which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering
Language: English
Record Identifier: 99450981002621
Output Type: Journal article

Metrics

4 File views/ downloads

139 Record Views

53 Times Cited - Web of Science

See more details

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Chemistry, Multidisciplinary; Chemistry, Physical; Materials Science, Multidisciplinary; Nanoscience & Nanotechnology

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

Source: InCites