Journal article
Comparison of nine antigen detection kits for diagnosis of urogenital infections due to Chlamydia psittaci in koalas
Journal of Clinical Microbiology, Vol.30(12), pp.3200-3205
1992
Abstract
Chlamydia psittaci is the major cause of infectious disease in the koala (Phascolarctos cinereus). It causes four disease syndromes in the koala, namely, conjunctivitis, rhinitis, cystitis, and infertility (females only). Diagnosis of chlamydial infections in koalas relies primarily on isolation of the organism in cell culture. Serology has generally not been useful, and little use has previously been made of the commercially available antigen detection kits. We examined the sensitivity, specificity, and usefulness of three direct fluorescent-antibody kits (Vet-IF [Cell Labs], IMAGEN [Celltech], Chlamydia-Direct IF [Bio Merieux]) and six antigen detection enzyme-linked immunosorbent assay (ELISA) kits (Clearview [Unipath], Surecell [Kodak], Pathfinder [Kallestad], Chlamydia-EIA [Pharmacia], Chlamydiazyme [Abbott], IDEIA [Celltech]) for the detection of urogenital infections in koalas. Laboratory studies showed that the direct fluorescent-antibody kits were the least sensitive in this case and did not detect fewer than 10(4) elementary bodies per ml, while most ELISA kits detected between 130 and 600 elementary bodies per ml. Field study results showed that the Clearview kit was the most sensitive (91%) compared with the IDEIA (88%) and the Surecell (73%) kits. All three kits were more sensitive than cell culture (36%), highlighting viability loss problems that occur during transport. This study showed that the Clearview kit is sensitive, specific, and easy to use for the detection of type II (urogenital) C. psittaci from koalas in the field and warrants further evaluation.
Details
- Title
- Comparison of nine antigen detection kits for diagnosis of urogenital infections due to Chlamydia psittaci in koalas
- Authors
- M Wood (Author) - Queensland University of TechnologyPeter Timms (Author) - Queensland University of Technology
- Publication details
- Journal of Clinical Microbiology, Vol.30(12), pp.3200-3205
- Publisher
- American Society for Microbiology
- Date published
- 1992
- DOI
- 10.1128/JCM.30.12.3200-3205.1992
- ISSN
- 0095-1137
- Copyright note
- Copyright © 1992 American Society for Microbiology. Reproduced here in accordance with the publishers copyright policy.
- Organisation Unit
- University of the Sunshine Coast, Queensland; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99449990102621
- Output Type
- Journal article
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