Comparison of antigen detection and quantitative PCR in the detection of chlamydial infection in koalas (Phascolarctos cinereus)

Jon Hanger; J Loader; C Wan; K W Beagley; Peter Timms; Adam Polkinghorne

doi:10.1016/j.tvjl.2012.07.024

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Comparison of antigen detection and quantitative PCR in the detection of chlamydial infection in koalas (Phascolarctos cinereus)

Journal article

Open access

Peer reviewed

Comparison of antigen detection and quantitative PCR in the detection of chlamydial infection in koalas (Phascolarctos cinereus)

Jon Hanger, J Loader, C Wan, K W Beagley, Peter Timms and Adam Polkinghorne

The Veterinary Journal, Vol.195(3), pp.391-393

2013

DOI: https://doi.org/10.1016/j.tvjl.2012.07.024

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Abstract

Chlamydia pecorum

antigen detection

quantitative PCR

koala

The gold standard method for detecting chlamydial infection in domestic and wild animals is PCR, but the technique is not suited to testing animals in the field when a rapid diagnosis is frequently required. The objective of this study was to compare the results of a commercially available enzyme immunoassay test for Chlamydia against a quantitative Chlamydia pecorum-specific PCR performed on swabs collected from the conjunctival sac, nasal cavity and urogenital sinuses of naturally infected koalas (Phascolarctos cinereus).The level of agreement for positive results between the two assays was low (43.2%). The immunoassay detection cut-off was determined as approximately 400 C. pecorum copies, indicating that the test was sufficiently sensitive to be used for the rapid diagnosis of active chlamydial infections.

Details

Title: Comparison of antigen detection and quantitative PCR in the detection of chlamydial infection in koalas (Phascolarctos cinereus)
Authors: Jon Hanger (Author) - Australia Zoo Wildlife Hospital
J Loader (Author) - Australia Zoo Wildlife Hospital
C Wan (Author) - Queensland University of Technology
K W Beagley (Author) - Queensland University of Technology
Peter Timms (Author) - Queensland University of Technology
Adam Polkinghorne (Author) - Queensland University of Technology
Publication details: The Veterinary Journal, Vol.195(3), pp.391-393
Publisher: Elsevier Ltd.
Date published: 2013
DOI: 10.1016/j.tvjl.2012.07.024
ISSN: 1090-0233
Organisation Unit: University of the Sunshine Coast, Queensland; Centre for Bioinnovation
Language: English
Record Identifier: 99449030702621
Output Type: Journal article

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