Journal article
Comparison of antigen detection and quantitative PCR in the detection of chlamydial infection in koalas (Phascolarctos cinereus)
The Veterinary Journal, Vol.195(3), pp.391-393
2013
Abstract
The gold standard method for detecting chlamydial infection in domestic and wild animals is PCR, but the technique is not suited to testing animals in the field when a rapid diagnosis is frequently required. The objective of this study was to compare the results of a commercially available enzyme immunoassay test for Chlamydia against a quantitative Chlamydia pecorum-specific PCR performed on swabs collected from the conjunctival sac, nasal cavity and urogenital sinuses of naturally infected koalas (Phascolarctos cinereus).The level of agreement for positive results between the two assays was low (43.2%). The immunoassay detection cut-off was determined as approximately 400 C. pecorum copies, indicating that the test was sufficiently sensitive to be used for the rapid diagnosis of active chlamydial infections.
Details
- Title
- Comparison of antigen detection and quantitative PCR in the detection of chlamydial infection in koalas (Phascolarctos cinereus)
- Authors
- Jon Hanger (Author) - Australia Zoo Wildlife HospitalJ Loader (Author) - Australia Zoo Wildlife HospitalC Wan (Author) - Queensland University of TechnologyK W Beagley (Author) - Queensland University of TechnologyPeter Timms (Author) - Queensland University of TechnologyAdam Polkinghorne (Author) - Queensland University of Technology
- Publication details
- The Veterinary Journal, Vol.195(3), pp.391-393
- Publisher
- Elsevier Ltd.
- Date published
- 2013
- DOI
- 10.1016/j.tvjl.2012.07.024
- ISSN
- 1090-0233; 1090-0233
- Copyright note
- Copyright © 2013. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
- Organisation Unit
- University of the Sunshine Coast, Queensland; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99449030702621
- Output Type
- Journal article
- Research Statement
- false
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