Comparison of TaqMan PCR assays for detection of the melioidosis agent Burkholderia pseudomallei in clinical specimens

M Kaestli; L J Richardson; R E Colman; A Tuanyok; Erin P Price; J R Bowers; M Mayo; E Kelley; M L Seymour; Derek S Sarovich; T Pearson; D M Engelthaler; D M Wagner; P S Keim; J M Schupp; B J Currie

doi:10.1128/JCM.06737-11

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Comparison of TaqMan PCR assays for detection of the melioidosis agent Burkholderia pseudomallei in clinical specimens

Journal article

Open access

Peer reviewed

Comparison of TaqMan PCR assays for detection of the melioidosis agent Burkholderia pseudomallei in clinical specimens

M Kaestli, L J Richardson, R E Colman, A Tuanyok, Erin P Price, J R Bowers, M Mayo, E Kelley, M L Seymour, Derek S Sarovich, …

Journal of Clinical Microbiology, Vol.50(6), pp.2059-2062

2012

DOI: https://doi.org/10.1128/JCM.06737-11

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Abstract

Melioidosis is an emerging infectious disease caused by the soil bacterium Burkholderia pseudomallei. In diagnostic and forensic settings, molecular detection assays need not only high sensitivity with low limits of detection but also high specificity. In a direct comparison of published and newly developed TaqMan PCR assays, we found the TTS1-orf2 assay to be superior in detecting B. pseudomallei directly from clinical specimens. The YLF/BTFC multiplex assay (targeting the Yersinia-like fimbrial/Burkholderia thailandensis-like flagellum and chemotaxis region) also showed high diagnostic sensitivity and provides additional information on possible geographic origin. Copyright © 2012, American Society for Microbiology. All Rights Reserved.

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Title: Comparison of TaqMan PCR assays for detection of the melioidosis agent Burkholderia pseudomallei in clinical specimens
Authors: M Kaestli (Author) - Charles Darwin University
L J Richardson (Author) - Charles Darwin University
R E Colman (Author) - Translational Genomics Research Institute, United States
A Tuanyok (Author) - Northern Arizona University, United States
Erin P Price (Author) - Northern Arizona University, United States
J R Bowers (Author) - Translational Genomics Research Institute, United States
M Mayo (Author) - Charles Darwin University
E Kelley (Author) - Translational Genomics Research Institute, United States
M L Seymour (Author) - Northern Arizona University, United States
Derek S Sarovich (Author) - Northern Arizona University, United States
T Pearson (Author) - Northern Arizona University, United States
D M Engelthaler (Author) - Translational Genomics Research Institute, United States
D M Wagner (Author) - Northern Arizona University, United States
P S Keim (Author) - Northern Arizona University, United States
J M Schupp (Author) - Translational Genomics Research Institute, United States
B J Currie (Author) - Charles Darwin University
Publication details: Journal of Clinical Microbiology, Vol.50(6), pp.2059-2062
Publisher: American Society for Microbiology
Date published: 2012
DOI: 10.1128/JCM.06737-11
ISSN: 0095-1137
Organisation Unit: University of the Sunshine Coast, Queensland; Centre for Bioinnovation
Language: English
Record Identifier: 99451286102621
Output Type: Journal article

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Web Of Science research areas: Microbiology

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