Journal article
Comparison of PTCH1, COX-2, p53, and Ki-67 protein expression in basal cell carcinomas of nodular and superficial subtypes arising on the head and trunk
International Journal of Dermatology, Vol.55(10), pp.1096-1105
2016
Abstract
Background: There is some evidence that basal cell carcinomas (BCCs) arising on different anatomic sites and developing to different histological subtypes differ in their pathophysiology. The expression of a number of proteins, including PTCH1, COX-2, p53, and Ki-67, is frequently altered in BCC development. Objectives: This study sought to determine whether protein expression differs between BCCs at different anatomic sites and of different histological subtypes. Methods: Expression of PTCH1, COX-2, p53, and Ki-67 proteins was compared between: (i) BCCs arising on the head (n = 55) and trunk (n = 53), and (ii) nodular (n = 52) and superficial (n = 43) BCCs. The intensity of immunohistochemistry (IHC) staining (low, moderate, strong, very strong) for PTCH1 and COX-2 proteins was measured and the proportions of p53- and Ki-67-positive cells quantified. Results: The proportion of cells expressing Ki-67 was higher in tumor tissue than in non-malignant epidermis, whereas the opposite was found for PTCH1. The IHC staining intensity for PTCH1 was substantially greater in truncal BCCs than in BCCs on the head (odds ratio [OR] 3.82, 95% confidence interval [CI] 1.63-8.96). The intensity of staining for PTCH1 was greater for superficial than for nodular BCCs (OR 3.70, 95% CI 1.53-8.97), and superficial BCCs showed a higher proportion of Ki-67-positive cells (OR 5.57, 95% CI 1.66-18.67). Conclusions: These differences suggest that the pathophysiology of BCC differs between lesions on the head and trunk and between nodular and superficial subtypes, perhaps indicating differences in their etiology.
Details
- Title
- Comparison of PTCH1, COX-2, p53, and Ki-67 protein expression in basal cell carcinomas of nodular and superficial subtypes arising on the head and trunk
- Authors
- Mohammad Khalesi (Author) - Queensland University of TechnologyMary Waterhouse (Author) - QIMR Berghofer Medical Research InstituteDavid C Whiteman (Author) - QIMR Berghofer Medical Research InstituteRichard Johns (Author) - Skin Cancer College AustralasiaCliff Rosendahl (Author) - University of QueenslandTimothy Hackett (Author) - University of QueenslandThomas Pollak (Author) - University of QueenslandMichael G Kimlin (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringElke Hacker (Author) - Queensland University of TechnologyRachel E Neale (Author) - QIMR Berghofer Medical Research Institute
- Publication details
- International Journal of Dermatology, Vol.55(10), pp.1096-1105
- Publisher
- Wiley-Blackwell Publishing Ltd.
- Date published
- 2016
- DOI
- 10.1111/ijd.13276
- ISSN
- 0011-9059
- Organisation Unit
- University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy
- Language
- English
- Record Identifier
- 99450128702621
- Output Type
- Journal article
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- Domestic collaboration
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- Dermatology
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Source: InCites