Journal article
Comparison of Concentration Methods for Rapid Detection of Hookworm Ova in Wastewater Matrices Using Quantitative PCR
Experimental Parasitology, Vol.159, pp.160-167
2015
Abstract
Hookworm infection contributes around 700 million infections worldwide especially in developing nations due to poor sanitation. The effective recovery of hookworm ova from wastewater matrices is difficult due to their low concentrations and heterogeneous distribution. In this study, we compared the recovery rates of (i) four rapid hookworm ova concentration methods from municipal wastewater, and (ii) two concentration methods from sludge samples. Ancylostoma caninum ova were used as surrogate for human hookworm (Ancylostoma duodenale and Necator americanus). Known concentration of A. caninum hookworm ova were seeded into wastewater (treated and raw) and sludge samples collected from two wastewater treatment plants (WWTPs) in Brisbane and Perth, Australia. The A. caninum ova were concentrated from treated and raw wastewater samples using centrifugation (Method A), hollow fiber ultrafiltration (HFUF) (Method B), filtration (Method C) and flotation (Method D) methods. For sludge samples, flotation (Method E) and direct DNA extraction (Method F) methods were used. Among the four methods tested, filtration (Method C) method was able to recover higher concentrations of A. caninum ova consistently from treated wastewater (39-50%) and raw wastewater (7.1-12%) samples collected from both WWTPs. The remaining methods (Methods A, B and D) yielded variable recovery rates ranging from 0.2 to 40% for treated and raw wastewater samples. The recovery rates for sludge samples were poor (0.02-4.7), although, Method F (direct DNA extraction) provided 1-2 orders of magnitude higher recovery rate than Method E (flotation). Based on our results it can be concluded that the recovery rates of hookworm ova from wastewater matrices, especially sludge samples, can be poor and highly variable. Therefore, choice of concentration method is vital for the sensitive detection of hookworm ova in wastewater matrices.
Details
- Title
- Comparison of Concentration Methods for Rapid Detection of Hookworm Ova in Wastewater Matrices Using Quantitative PCR
- Authors
- P Gyawali (Author) - University of QueenslandWarish Ahmed (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringP Jagals (Author)Jatinder P S Sidhu (Author) - University of QueenslandS Toze (Author) - University of Queensland
- Publication details
- Experimental Parasitology, Vol.159, pp.160-167
- Publisher
- Academic Press
- Date published
- 2015
- DOI
- 10.1016/j.exppara.2015.09.002
- ISSN
- 0014-4894
- Copyright note
- Copyright © 2015. This manuscript version is made available under the CC-BY-NC-ND 4.0 licence http://creativecommons.org/licences/by-nc-nd/4.0/ The definitive version is available from http://www.dx.doi.org/10.1016/j.exppara.2015.09.002
- Organisation Unit
- University of the Sunshine Coast, Queensland
- Language
- English
- Record Identifier
- 99449575102621
- Output Type
- Journal article
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