Journal article
Comparison between virulence characteristics of dominant and non-dominant Escherichia coli strains of the gut and their interaction with Caco-2 cells
Microbial Pathogenesis, Vol.105, pp.171-176
2017
Abstract
Escherichia coli strains are normal inhabitants of the gut and are normally found in the faeces of the host at different population sizes. We characterised faecal E. coli of 45 healthy male (n = 17) and female (n = 28) volunteers by testing 28 isolates from each individual. These isolates were typed and divided into dominant (if constituted >50% of the population tested) and non-dominant types in each individual. Representative strains of each dominant and non-dominant type were tested for their virulence gene profiles, their ability to form biofilm, adhere to, invade and translocate through a gut epithelial cell line (Caco-2 cells). Strains belonging to dominant types adhered significantly more to Caco-2 cells than non-dominant strains (5.7±0.3 versus 4.3.± 0.13 CFU/cell mean±SEM, P = 0.0003). They also invaded (135±6 versus 63±13 CFU) and translocated through Caco-2 cells (84±5 versus 32±9 CFU) significantly more than non-dominant strains (P < 0.0001 and P = 0.0002, respectively). Moreover, dominant strains showed the ability to form significantly more biofilm than non-dominant strains (1.1±0.01 versus 0.5±0.1 OD600, P < 0.0001). Majority (51%) of the strains belonged to phylogroup D followed by B2 (23%). Furthermore, out of 25 virulence genes tested, kpsMTII, papC and papG allele III were found to be significantly higher among dominant than non-dominant strains. Our results suggest that E. coli strains dominating the gut may have virulence properties that enable them to efficiently interact with the gut epithelium and translocate under predisposing conditions of the host.
Details
- Title
- Comparison between virulence characteristics of dominant and non-dominant Escherichia coli strains of the gut and their interaction with Caco-2 cells
- Authors
- Bahareh Owrangi (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringNicole Masters (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringTara L Vollmerhausen (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringChristian A O'Dea (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringAnna V Kuballa (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringMohammad Katouli (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
- Publication details
- Microbial Pathogenesis, Vol.105, pp.171-176
- Publisher
- Academic Press
- Date published
- 2017
- DOI
- 10.1016/j.micpath.2017.02.032
- ISSN
- 0882-4010
- Organisation Unit
- School of Health - Biomedicine; School of Science and Engineering - Legacy; School of Health; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy; School of Health and Sport Sciences - Legacy; Forest Industries Research Centre; School of Science, Technology and Engineering; Centre for Bioinnovation; School of Health and Behavioural Sciences - Legacy
- Language
- English
- Record Identifier
- 99451298802621
- Output Type
- Journal article
Metrics
8 File views/ downloads
1145 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- International collaboration
- Web Of Science research areas
- Immunology
- Microbiology
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites