Journal article
Comparing different techniques for obtaining molecular size distributions of glycogen
European Polymer Journal, Vol.201, pp.1-7
2023
Abstract
Glycogen is a hyperbranched glucose polymer, serving as a major energy reservoir in animals. Characterization of the size distributions of complex branched polysaccharides helps in the understanding of their structural and functional properties. This study compared the effectiveness of various techniques in analyzing glycogen size distributions, namely size-separation-based techniques (size exclusion chromatography (SEC) and asymmetric-flow field-flow fractionation (AF4)) and imaging-based methods (transmission electron microscopy (TEM) and atomic force microscopy (AFM)). SEC and AF4 gave similar glycogen size distributions. TEM was suboptimal for obtaining size distributions due to the difficulty of preventing aggregation artifacts during sample preparation. AFM appeared to avoid the problem, but the size distributions so obtained had substantially lower resolution and were much more laborious to acquire. The microscopy-based techniques, especially TEM, do however provide richer morphological information on individual particles than do SEC and AF4. These results suggest that combining size-separation and microscopy (imaging) techniques could provide a holistic view of glycogen size distribution and morphology, which may also be applicable to the characterization of other complex branched polymers.
Details
- Title
- Comparing different techniques for obtaining molecular size distributions of glycogen
- Authors
- Yujun Wan - University of QueenslandSheena M.H. Chua - University of QueenslandYin Yao - UNSW SydneyLewis Adler - UNSW SydneyMarta Navarro - University of QueenslandEugeni Roura - University of QueenslandRichard D. Tilley - UNSW SydneyChangfeng Li - Yangzhou UniversityLars Nilsson - Lund UniversityRobert G Gilbert (Corresponding Author) - University of QueenslandMitchell A Sullivan (Corresponding Author) - University of Queensland
- Publication details
- European Polymer Journal, Vol.201, pp.1-7
- Publisher
- Elsevier Ltd
- Date published
- 2023
- DOI
- 10.1016/j.eurpolymj.2023.112518
- ISSN
- 1873-1945
- Organisation Unit
- School of Health - Biomedicine
- Language
- English
- Record Identifier
- 991035092702621
- Output Type
- Journal article
Metrics
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web Of Science research areas
- Polymer Science