Comparative proteomic study reveals the enhanced immune response with the blockade of interleukin 10 with anti-IL-10 and anti-IL-10 receptor antibodies in human U937 cells

Guoying Ni; Shu Chen; Jianwei Yuan; Shelley F Cavezza; Ming Q Wei; Hejie Li; Xuan Pan; Xiao Song Liu; Tianfang Wang

doi:10.1371/journal.pone.0213813

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Comparative proteomic study reveals the enhanced immune response with the blockade of interleukin 10 with anti-IL-10 and anti-IL-10 receptor antibodies in human U937 cells

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Comparative proteomic study reveals the enhanced immune response with the blockade of interleukin 10 with anti-IL-10 and anti-IL-10 receptor antibodies in human U937 cells

Guoying Ni, Shu Chen, Jianwei Yuan, Shelley F Cavezza, Ming Q Wei, Hejie Li, Xuan Pan, Xiao Song Liu and Tianfang Wang

PLoS One, Vol.14(3), e0213813

2019

DOI: https://doi.org/10.1371/journal.pone.0213813

PMCID: PMC6428271

PMID: 30897137

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Abstract

Blocking cytokine interleukin 10 (IL-10) at the time of immunisation enhances vaccine induced T cell responses and improves control of tumour cell growth in vivo. However, the effect of an IL-10 blockade on the biological function of macrophages has not been explored. In the current paper, a macrophage precursor cell line, U937 cells, was selected to investigate the differential expression of proteins and relevant cell signalling pathway changes, when stimulated with lipopolysaccharide (LPS) in the presence of antibodies to IL-10 or IL-10 receptor. We used a quantitative proteomic strategy to investigate variations in protein profiles of U937 cells following the treatments with LPS, LPS plus human anti-IL10 antibody and anti-IL10R antibody in 24hrs, respectively. The LPS treatment significantly activated actin-related cell matrix formation and immune response pathways. The addition of anti-IL10 and anti-IL10R antibody further promoted the immune response and potentially effect macrophage survival through PI3K/AKT signalling; however, the latter appeared to also upregulated oncogene XRCC5 and Cajal body associated processes.

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Title: Comparative proteomic study reveals the enhanced immune response with the blockade of interleukin 10 with anti-IL-10 and anti-IL-10 receptor antibodies in human U937 cells
Authors: Guoying Ni (Author) - University of the Sunshine Coast
Shu Chen (Author) - Cancer Research Institute, China
Jianwei Yuan (Author) - Guangdong Pharmaceutical University, China
Shelley F Cavezza (Author) - University of the Sunshine Coast
Ming Q Wei (Author) - Griffith University
Hejie Li (Author) - University of Tokyo, Japan
Xuan Pan (Author) - Guangdong Pharmaceutical University, China
Xiao Song Liu (Author) - University of the Sunshine Coast
Tianfang Wang (Author) - University of the Sunshine Coast
Publication details: PLoS One, Vol.14(3), e0213813
Publisher: Public Library of Science
Date published: 2019
DOI: 10.1371/journal.pone.0213813
ISSN: 1932-6203
PMID: 30897137; PMC6428271
Copyright note: Copyright © 2019 Ni et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy; School of Health and Sport Sciences - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99451247902621
Output Type: Journal article

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Web Of Science research areas: Cell Biology

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