Journal article
Comparative proteomic study reveals the enhanced immune response with the blockade of interleukin 10 with anti-IL-10 and anti-IL-10 receptor antibodies in human U937 cells
PLoS One, Vol.14(3), e0213813
2019
Abstract
Blocking cytokine interleukin 10 (IL-10) at the time of immunisation enhances vaccine induced T cell responses and improves control of tumour cell growth in vivo. However, the effect of an IL-10 blockade on the biological function of macrophages has not been explored. In the current paper, a macrophage precursor cell line, U937 cells, was selected to investigate the differential expression of proteins and relevant cell signalling pathway changes, when stimulated with lipopolysaccharide (LPS) in the presence of antibodies to IL-10 or IL-10 receptor. We used a quantitative proteomic strategy to investigate variations in protein profiles of U937 cells following the treatments with LPS, LPS plus human anti-IL10 antibody and anti-IL10R antibody in 24hrs, respectively. The LPS treatment significantly activated actin-related cell matrix formation and immune response pathways. The addition of anti-IL10 and anti-IL10R antibody further promoted the immune response and potentially effect macrophage survival through PI3K/AKT signalling; however, the latter appeared to also upregulated oncogene XRCC5 and Cajal body associated processes.
Details
- Title
- Comparative proteomic study reveals the enhanced immune response with the blockade of interleukin 10 with anti-IL-10 and anti-IL-10 receptor antibodies in human U937 cells
- Authors
- Guoying Ni (Author) - University of the Sunshine CoastShu Chen (Author) - Cancer Research Institute, ChinaJianwei Yuan (Author) - Guangdong Pharmaceutical University, ChinaShelley F Cavezza (Author) - University of the Sunshine CoastMing Q Wei (Author) - Griffith UniversityHejie Li (Author) - University of Tokyo, JapanXuan Pan (Author) - Guangdong Pharmaceutical University, ChinaXiao Song Liu (Author) - University of the Sunshine CoastTianfang Wang (Author) - University of the Sunshine Coast
- Publication details
- PLoS One, Vol.14(3), e0213813
- Publisher
- Public Library of Science
- Date published
- 2019
- DOI
- 10.1371/journal.pone.0213813
- ISSN
- 1932-6203
- Copyright note
- Copyright © 2019 Ni et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
- Organisation Unit
- School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy; School of Health and Sport Sciences - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99451247902621
- Output Type
- Journal article
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