Journal article
Comparative genomics and antimicrobial resistance profiling of Elizabethkingia isolates reveals nosocomial transmission and in vitro susceptibility to fluoroquinolones, tetracyclines and trimethoprim-sulfamethoxazole
Journal of Clinical Microbiology, Vol.58(9), e00730-20
2020
PMCID: PMC7448627
PMID: 32580952
Abstract
The Elizabethkingia genus has gained global attention in recent years as a sporadic, worldwide, nosocomial pathogen. Elizabethkingia spp. are intrinsically multidrug resistant, primarily infect immunocompromised individuals, and are associated with high mortality (∼20-40%). Yet, gaps remain in our understanding of transmission, global strain relatedness, antimicrobial resistance and effective therapy. Over a 16-year period 22 clinical and six hospital environmental isolates were collected from Queensland, Australia. Identification using the MALDI-TOF MS (VITEK® MS) and whole-genome sequencing was compared with a global strain dataset. Phylogenomic reconstruction robustly identified 22 E. anophelis, three E. miricola, two E. meningoseptica and one E. bruuniana, most of which branched as unique lineages. Global analysisrevealed some Australian E. anophelis isolates are genetically closely related to strains identified from the USA, England and Asia. Comparative genomics of clinical and environmental strains identified evidence of nosocomial transmission in patients, indicating probable infection from a hospital reservoir. Furthermore, broth microdilution against 39 antimicrobials revealed almost ubiquitous resistance to aminoglycosides, carbapenems, cephalosporins and penicillins. Like other international strains, our isolates expressed susceptibility to minocycline and levofloxacin and the less common trimethoprim/sulfamethoxazole. Our study demonstrates important new insights into the genetic diversity, environmental persistence, transmission of and potential effective therapy for Australian Elizabethkingia species.
Details
- Title
- Comparative genomics and antimicrobial resistance profiling of Elizabethkingia isolates reveals nosocomial transmission and in vitro susceptibility to fluoroquinolones, tetracyclines and trimethoprim-sulfamethoxazole
- Authors
- Delaney Burnard (Author) - University of the Sunshine Coast, Queensland, GeneCology Research Centre - LegacyLetitia Gore (Author) - Queensland HealthAndrew Henderson (Author) - The University of QueenslandAma Ranasinghe (Author) - The University of QueenslandHaakon Bergh (Author) - Queensland HealthKyra Cottrell (Author) - The University of QueenslandDerek S Sarovich (Author) - University of the Sunshine Coast, Queensland, GeneCology Research Centre - LegacyErin P Price (Author) - University of the Sunshine Coast, Queensland, GeneCology Research Centre - LegacyDavid L Paterson (Author) - The University of QueenslandPatrick N. A Harris (Author) - The University of Queensland
- Publication details
- Journal of Clinical Microbiology, Vol.58(9), e00730-20
- Publisher
- American Society for Microbiology
- Date published
- 2020
- DOI
- 10.1128/JCM.00730-20
- ISSN
- 1098-660X
- PMID
- 32580952; PMC7448627
- Organisation Unit
- School of Science and Engineering - Legacy; Information Technology; University of the Sunshine Coast, Queensland; Centre for Bioinnovation; Sustainability Research Cluster
- Language
- English
- Record Identifier
- 99469907802621
- Output Type
- Journal article
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