Comparative Sequence Analysis of recA Gene among Vibrio cholerae Isolates from Iran with Globally reported sequences

A Dashtbani-Roozbehani; B Bakhshi; Mohammad Katouli; M R Pourshafie

doi:10.1111/j.1472-765X.2011.03108.x

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Comparative Sequence Analysis of recA Gene among Vibrio cholerae Isolates from Iran with Globally reported sequences

Journal article

Peer reviewed

Comparative Sequence Analysis of recA Gene among Vibrio cholerae Isolates from Iran with Globally reported sequences

A Dashtbani-Roozbehani, B Bakhshi, Mohammad Katouli and M R Pourshafie

Letters in Applied Microbiology, Vol.53(3), pp.313-323

2011

DOI: https://doi.org/10.1111/j.1472-765X.2011.03108.x

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Abstract

Vibrio cholerae

recA

sequence typing

housekeeping gene

Aims: To study the genetic relatedness between V.cholerae isolates from Iran and other countries based on housekeeping gene recA sequence analysis. Methods and Results: A 995 bp region of the recA gene from 24 V.cholerae isolates obtained from human and surface water origins in Iran over a five year period were sequenced and compared with the sequence data from the isolates belonging to other places. Cluster analysis of the constructed dendrogram based on recA sequence divergence for our clinical isolates showed one sequence type (ST), whereas environmental isolates revealed eight STs. Interestingly, one of our environmental isolates was intermixed with clinical isolates in the largest cluster containing the epidemic strains. Our 24 isolates plus 198 global isolates available in the GenBank showed 77 sequence types (STs) with at least one nucleotide difference. Conclusions: Our result suggested that recA sequencing is a reliable analysis method for understanding the relatedness of the local isolates with the isolates obtained elsewhere. Significance and Impact of Study: Understanding the genetic relatedness between V.cholerae isolates could give insights to the health care system for better control and prevention of the cholera.

Details

Title: Comparative Sequence Analysis of recA Gene among Vibrio cholerae Isolates from Iran with Globally reported sequences
Authors: A Dashtbani-Roozbehani (Author) - Pasteur Institute of Iran, Iran
B Bakhshi (Author) - Tarbiat Modares University, Iran
Mohammad Katouli (Author) - University of the Sunshine Coast - Faculty of Science, Health and Education
M R Pourshafie (Author) - Pasteur Institute of Iran, Iran
Publication details: Letters in Applied Microbiology, Vol.53(3), pp.313-323
Publisher: Wiley-Blackwell Publishing Ltd.
Date published: 2011
DOI: 10.1111/j.1472-765X.2011.03108.x
ISSN: 0266-8254
Organisation Unit: University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99449762402621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Biotechnology & Applied Microbiology; Microbiology

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