Comparative Proteomic Study of the Antiproliferative Activity of Frog Host-Defence Peptide Caerin 1.9 and Its Additive Effect with Caerin 1.1 on TC-1 Cells Transformed with HPV16 E6 and E7

Guoying Ni; Di Liang; Scott F Cummins; Shelley F Walton; Shu Chen; Yuejian Wang; Kate E Mounsey; Ming Q Wei; Jianwei Yuan; Xuan Pan; Xiao Song Liu; Tianfang Wang

doi:10.1155/2018/7382351

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Comparative Proteomic Study of the Antiproliferative Activity of Frog Host-Defence Peptide Caerin 1.9 and Its Additive Effect with Caerin 1.1 on TC-1 Cells Transformed with HPV16 E6 and E7

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Comparative Proteomic Study of the Antiproliferative Activity of Frog Host-Defence Peptide Caerin 1.9 and Its Additive Effect with Caerin 1.1 on TC-1 Cells Transformed with HPV16 E6 and E7

Guoying Ni, Di Liang, Scott F Cummins, Shelley F Walton, Shu Chen, Yuejian Wang, Kate E Mounsey, Ming Q Wei, Jianwei Yuan, Xuan Pan, …

BioMed Research International, Vol.2018, 7382351

2018

DOI: https://doi.org/10.1155/2018/7382351

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Abstract

Caerin is a family of peptides isolated from the glandular secretion of Australian tree frogs, the genus Litoria, and has been previously shown to have anticancer activity against several cancer cells. In this work, we used two host-defence peptides, caerin 1.1 and caerin 1.9, to investigate their ability to inhibit a murine derived TC-1 cell transformed with human papillomavirus 16 E6 and E7 growth in vitro. Caerin 1.9 inhibits TC-1 cell proliferation, although inhibition is more pronounced when applied in conjunction with caerin 1.1. To gain further insights into the antiproliferative mechanisms of caerin 1.9 and its additive effect with caerin 1.1, we used a proteomics strategy to quantitatively examine (i) the changes in the protein profiles of TC-1 cells and (ii) the excretory-secretory products of TC-1 cells following caerin peptides treatment. Caerin 1.9 treatment significantly altered the abundance of several immune-related proteins and related pathways, such as the Tec kinase and ILK signalling pathways, as well as the levels of proinflammatory cytokines and chemokines. In conclusion, caerin peptides inhibit TC-1 cell proliferation, associated with modification in signalling pathways that would change the tumour microenvironment which is normally immune suppressive.

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Title: Comparative Proteomic Study of the Antiproliferative Activity of Frog Host-Defence Peptide Caerin 1.9 and Its Additive Effect with Caerin 1.1 on TC-1 Cells Transformed with HPV16 E6 and E7
Authors: Guoying Ni (Author) - Guangdong Pharmaceutical University, China
Di Liang (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Scott F Cummins (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Shelley F Walton (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Shu Chen (Author) - First People's Hospital of Foshan, China
Yuejian Wang (Author) - First People's Hospital of Foshan, China
Kate E Mounsey (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Ming Q Wei (Author) - Griffith University
Jianwei Yuan (Author) - Guangdong Pharmaceutical University, China
Xuan Pan (Author) - Guangdong Pharmaceutical University, China
Xiao Song Liu (Author) - Guangdong Pharmaceutical University, China
Tianfang Wang (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Publication details: BioMed Research International, Vol.2018, 7382351; 14
Publisher: Hindawi Publishing Corporation
Date published: 2018
DOI: 10.1155/2018/7382351
ISSN: 2314-6133
Copyright note: Copyright © 2018 Guoying Ni et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Organisation Unit: School of Health - Biomedicine; School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy; School of Health and Sport Sciences - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation; School of Health and Behavioural Sciences - Legacy
Language: English
Record Identifier: 99450756502621
Output Type: Journal article

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Web Of Science research areas: Biotechnology & Applied Microbiology; Medicine, Research & Experimental

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