Journal article
Comparative Genomics of Burkholderia singularis sp. nov., a Low G+C Content, Free-Living Bacterium That Defies Taxonomic Dissection of the Genus Burkholderia
Frontiers in Microbiology, Vol.8, 1679
2017
Abstract
Four Burkholderia pseudomallei-like isolates of human clinical origin were examined by a polyphasic taxonomic approach that included comparative whole genome analyses. The results demonstrated that these isolates represent a rare and unusual, novel Burkholderia species for which we propose the name B. singularis. The type strain is LMG 28154T (=CCUG 65685T ). Its genome sequence has an average mol% G+C content of 64.34%, which is considerably lower than that of other Burkholderia species. The reduced G+C content of strain LMG 28154T was characterized by a genome wide AT bias that was not due to reduced GC-biased gene conversion or reductive genome evolution, but might have been caused by an altered DNA base excision repair pathway. B. singularis can be differentiated from other Burkholderia species by multilocus sequence analysis, MALDI-TOF mass spectrometry and a distinctive biochemical profile that includes the absence of nitrate reduction, a mucoid appearance on Columbia sheep blood agar, and a slowly positive oxidase reaction. Comparisons with publicly available whole genome sequences demonstrated that strain TSV85, an Australian water isolate, also represents the same species and therefore, to date, B. singularis has been recovered from human or environmental samples on three continents.
Details
- Title
- Comparative Genomics of Burkholderia singularis sp. nov., a Low G+C Content, Free-Living Bacterium That Defies Taxonomic Dissection of the Genus Burkholderia
- Authors
- Peter Vandamme (Author) - Ghent University, BelgiumCharlotte Peeters (Author) - Ghent University, BelgiumBirgit De Smet (Author) - Ghent University, BelgiumErin P Price (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringDerek S Sarovich (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringDeborah A Henry (Author) - University of British Columbia, CanadaTrevor J Hird (Author) - University of British Columbia, CanadaJames E A Zloznik (Author) - University of British Columbia, CanadaMark Mayo (Author) - Menzies School of Health ResearchJeffrey Warner (Author) - James Cook UniversityAnthony Baker (Author) - University of TasmaniaBart J Currie (Author) - Menzies School of Health ResearchAurelien Carlier (Author) - Ghent University, Belgium
- Publication details
- Frontiers in Microbiology, Vol.8, 1679; 14
- Publisher
- Frontiers Research Foundation
- Date published
- 2017
- DOI
- 10.3389/fmicb.2017.01679
- ISSN
- 1664-302X
- Copyright note
- Copyright © 2017 Vandamme, Peeters, De Smet, Price, Sarovich, Henry, Hird, Zlosnik, Mayo, Warner, Baker, Currie and Carlier. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms
- Organisation Unit
- University of the Sunshine Coast, Queensland; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99450332402621
- Output Type
- Journal article
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