Journal article
Commonality of adherent-invasive Escherichia coli isolated from patients with extraintestinal infections, healthy individuals and the environment
European Journal of Clinical Microbiology & Infectious Diseases, Vol.40(1), pp.181-192
2021
PMID: 33063232
Abstract
Adherent-invasive Escherichia coli (AIEC) has been implicated as a microbiological factor in inflammatory bowel disease (IBD) pathogenesis. These strains are defined by their ability to adhere to and invade intestinal epithelial cells, and to survive and replicate in macrophages. We postulated that AIEC strains may commonly inhabit the gut of healthy individuals (HI), cause extraintestinal infections, and be found in sewage treatment plants (STP) and surface waters (SW). A total of 808 E. coli strains isolated from HI; patients with community-acquired urinary tract infection (CA-UTI), septicaemia and urosepsis; STP; and SW, showing a diffuse adhesion pattern to Caco-2 cells were included in this study. Typing of the strains using a combination of RAPD-PCR and PhPlate fingerprinting grouped them into 48 common clones (CCs). Representatives of each CC were tested for the ability to invade Caco-2 cells, survive and replicate in macrophages, and for the presence of six virulence genes commonly found among AIEC strains. Twenty CCs were deemed AIEC based on their ability to survive and replicate in macrophages, while encoding htrA, dsbA and clbA genes. These CCs primarily originated from HI and CA-UTI patients but were also detected in secondary locations including STP and SW. Strains lacking intramacrophagic survival and replication abilities were regarded as diffusely adhering E. coli (DAEC). Certain clones of AIEC are common in the gut of HI whilst promoting CA-UTI. The survival and persistence of AIEC in STP and SW may have serious public health ramifications for individuals predisposed to IBD.
Details
- Title
- Commonality of adherent-invasive Escherichia coli isolated from patients with extraintestinal infections, healthy individuals and the environment
- Authors
- Dylan Astley (Author) - University of the Sunshine Coast, Queensland, School of Science and Engineering - LegacyNicole Masters (Author) - University of the Sunshine Coast, Queensland, School of Health and Sport Sciences - LegacyAnna Kuballa (Author) - University of the Sunshine Coast, Queensland, School of Health and Sport Sciences - LegacyMohammad Katouli (Corresponding Author) - University of the Sunshine Coast, Queensland, School of Health and Sport Sciences - Legacy
- Publication details
- European Journal of Clinical Microbiology & Infectious Diseases, Vol.40(1), pp.181-192
- Publisher
- Springer
- Date published
- 2021
- DOI
- 10.1007/s10096-020-04066-5
- ISSN
- 1435-4373
- PMID
- 33063232
- Organisation Unit
- School of Health - Biomedicine; School of Science and Engineering - Legacy; School of Health; University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation; School of Health and Behavioural Sciences - Legacy
- Language
- English
- Record Identifier
- 99482292802621
- Output Type
- Journal article
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- Infectious Diseases
- Microbiology
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