Journal article
Come together: calibration of tiger shark (Galeocerdo cuvier) microsatellite databases for investigating global population structure and assignment of historical specimens
Conservation Genetics Resources, Vol.13(2), pp.209-220
2021
Abstract
This study provides the first standardized global microsatellite database for a shark species, the tiger shark (Galeocerdo cuvier). Genotyping of reference individuals was used to develop and apply a calibration key for data from eight microsat-ellite loci data produced by three different laboratories, thereby allowing merging of genotypes into a single dataset. The unified data helped to elucidate the global population structure of the species and provided improved statistical power, through allowing a higher number of samples per location compared to the original studies from which the samples were obtained. Pairwise F ST estimates and PCA plots showed significant genetic differentiation between Atlantic and Indo-Pacific samples, confirming previous findings by identifying the presence of a strong genetic break between tiger sharks inhabiting the two ocean basins. In turn, the standardized database (n = 799) also allowed archived historical samples to be genotyped and assigned back to their population (ocean basin) of origin. We demonstrate how calibration tests in population structure studies using microsatellite data is important as it simply provides more data to single studies. Importance factors for successful assignment analysis is discussed, as well as how the possibility of assigning historical samples of unknown origin back to the population, increases sample value. Our results demonstrate that global calibration of microsatellite and other genetic datasets can improve the statistical power and resolution of population structure analysis; an approach applicable not only when working with highly mobile globally distributed species such as the tiger shark, but with any species for which multiple genetic datasets exist.
Details
- Title
- Come together: calibration of tiger shark (Galeocerdo cuvier) microsatellite databases for investigating global population structure and assignment of historical specimens
- Authors
- M Sort (Corresponding Author) - Technical University of DenmarkA Manuzzi (Author) - Technical University of DenmarkB Jiménez-Mena (Author) - Technical University of DenmarkJennifer R Ovenden (Author) - University of QueenslandBonnie Holmes (Author) - University of the Sunshine Coast, Queensland, School of Science, Technology and EngineeringA M Bernard (Author) - Nova Southeastern UniversityM S Shivji (Author) - Nova Southeastern UniversityD Meldrup (Author) - Technical University of DenmarkMichael B Bennett (Author) - University of QueenslandEinar Eg Nielsen (Author) - Technical University of Denmark
- Publication details
- Conservation Genetics Resources, Vol.13(2), pp.209-220
- Publisher
- Springer Netherlands
- DOI
- 10.1007/s12686-021-01197-5
- ISSN
- 1877-7260
- Organisation Unit
- University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering
- Language
- English
- Record Identifier
- 99542508702621
- Output Type
- Journal article
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- Biodiversity Conservation
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