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Combined Diffusion Tensor Imaging and Quantitative Susceptibility Mapping Discern Discrete Facets of White Matter Pathology Post-injury in the Rodent 2.18 MB
CC BY V4.0, Open Access
Journal article
Combined Diffusion Tensor Imaging and Quantitative Susceptibility Mapping Discern Discrete Facets of White Matter Pathology Post-injury in the Rodent Brain
Frontiers in Neurology, Vol.11, pp.1-16
2020
PMCID: PMC7067826
PMID: 32210907
Abstract
Early loss of white matter microstructure integrity is a significant cause of long-term neurological disorders following traumatic brain injury (TBI). White matter abnormalities typically involve axonal loss and demyelination.
In-vivo
imaging tools to detect and differentiate such microstructural changes are not well-explored. This work utilizes the conjoint potential offered by advanced magnetic resonance imaging techniques, including quantitative susceptibility mapping (QSM) and diffusion tensor imaging (DTI), to discern the underlying white matter pathology at specific time points (5 h, 1, 3, 7, 14, and 30 days) post-injury in the controlled cortical impact mouse model. A total of 42 animals were randomized into six TBI groups (
n
= 6 per group) and one sham group (
n
= 6). Histopathology was performed to validate
in-vivo
findings by performing myelin basic protein (MBP) and glial fibrillary acidic protein (GFAP) immunostaining for the assessment of changes to myelin and astrocytes. After 5 h of injury radial diffusivity (RD) was increased in white matter without a significant change in axial diffusivity (AxD) and susceptibility values. After 1 day post-injury RD was decreased. AxD and susceptibility changes were seen after 3 days post-injury. Susceptibility increases in white matter were observed in both ipsilateral and contralateral regions and persisted for 30 days. In histology, an increase in GFAP immunoreactivity was observed after 3 days post-injury and remained high for 30 days in both ipsilateral and contralateral white matter regions. A loss in MBP signal was noted after 3 days post-injury that continued up to 30 days. In conclusion, these results demonstrate the complementary ability of DTI and QSM in discerning the micro-pathological processes triggered following TBI. While DTI revealed acute and focal white matter changes, QSM mirrored the temporal demyelination in the white matter tracts and diffuse regions at the chronic state.
Details
- Title
- Combined Diffusion Tensor Imaging and Quantitative Susceptibility Mapping Discern Discrete Facets of White Matter Pathology Post-injury in the Rodent Brain
- Authors
- Neha Soni (Author) - The University of QueenslandViktor Vegh (Author) - The University of QueenslandXuan Vinh To (Author) - The University of QueenslandAbdalla Z Mohamed (Author) - The University of QueenslandKarin Borges (Author) - The University of QueenslandFatima A Nasrallah (Corresponding Author) - The University of Queensland
- Publication details
- Frontiers in Neurology, Vol.11, pp.1-16
- Publisher
- Frontiers Research Foundation
- Date published
- 2020
- DOI
- 10.3389/fneur.2020.00153
- ISSN
- 1664-2295
- PMID
- 32210907; PMC7067826
- Copyright note
- Copyright © 2020 Soni, Vegh, To, Mohamed, Borges and Nasrallah. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
- Organisation Unit
- University of the Sunshine Coast, Queensland; Thompson Institute
- Language
- English
- Record Identifier
- 99525808802621
- Output Type
- Journal article
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