Combined Bioaugmentation and Biostimulation to Cleanup Soil Contaminated with High Concentrations of Atrazine

E Silva; A M Fialho; I Sa-Correia; Richard G Burns; L J Shaw

doi:10.1021/es0300822

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Combined Bioaugmentation and Biostimulation to Cleanup Soil Contaminated with High Concentrations of Atrazine

Journal article

Peer reviewed

Combined Bioaugmentation and Biostimulation to Cleanup Soil Contaminated with High Concentrations of Atrazine

E Silva, A M Fialho, I Sa-Correia, Richard G Burns and L J Shaw

Environmental Science and Technology, Vol.38(2), pp.632-637

2004

DOI: https://doi.org/10.1021/es0300822

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Abstract

bioremediation

cells

contamination

herbicides

soils

We developed a joint bioaugmentation and biostimulation approach for the clean up of soil contaminated with high (168.7 and 337.4 ?g g-1) concentrations of the herbicide atrazine (2-chloro-4-(ethylamino)-6-isopropylamino-s-triazine). Pseudomonas sp. strain ADP (P. ADP) was used for bioaugmentation (?107 cells g -1 soil), and citrate (concentration range 5.8-40 mg g-1 soil) and succinate (6.2-30.8 mg g-1]) were used for biostimulation. The study soil had indigenous potential for atrazine mineralization (54.4±2% of 168.7 ?g-1 mineralized after 67 day), but rapid mineralization only took place after a prolonged acclimation phase (?28 days). Inoculation with P. ADP alone resulted in a limited improvement in mineralization (e.g., 30.6±1% mineralization of 168.7 ?g g -1 of atrazine in inoculated soil cf. <0.5% in noninoculated in 7 days). Quantification of surviving numbers of P. ADP revealed a 10-fold decline from initial levels. However, bioaugmentation together with citrate or succinate biostimulation markedly increased P. ADP cell survival and atrazine mineralization (e.g., addition of 11.6 mg g-1 of citrate increased mineralization of 337.4 ?g g-1 of atrazine from <2 to 79.9±1% in 13 days). A critical parameter in determining the extent of atrazine mineralization by P. ADP was Cs:Natz (soluble carbon to atrazine nitrogen ratio): Cs:Natz > 40 was required for maximal atrazine mineralization. We suggest our observations may be used as a framework for rational bioremediation of field soils contaminated with atrazine.

Details

Title: Combined Bioaugmentation and Biostimulation to Cleanup Soil Contaminated with High Concentrations of Atrazine
Authors: E Silva (Author) - University of Kent, United Kingdom
A M Fialho (Author) - University of Kent, United Kingdom
I Sa-Correia (Author) - University of Kent, United Kingdom
Richard G Burns (Author) - University of Kent, United Kingdom
L J Shaw (Author) - University of Kent, United Kingdom
Publication details: Environmental Science and Technology, Vol.38(2), pp.632-637
Publisher: American Chemical Society
Date published: 2004
DOI: 10.1021/es0300822
ISSN: 0013-936X; 0013-936X
Organisation Unit: University of the Sunshine Coast, Queensland; Office of the Deputy Vice-Chancellor (Research and Innovation)
Language: English
Record Identifier: 99449369202621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Engineering, Environmental; Environmental Sciences

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