Journal article
Cloning of the crustacean hyperglycemic hormone and evidence for molt-inhibiting hormone within the central nervous system of the blue crab Portunus pelagicus
Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology, Vol.164(2), pp.276-290
2013
Abstract
The crustacean X-organ-sinus gland (XO-SG) complex controls molt-inhibiting hormone (MIH) production, although extra expression sites for MIH have been postulated. Therefore, to explore the expression of MIH and distinguish between the crustacean hyperglycemic hormone (CHH) superfamily, and MIH immunoreactive sites (ir) in the central nervous system (CNS), we cloned a CHH gene sequence for the crab Portunus pelagicus (Ppel-CHH), and compared it with crab CHHtype I and II peptides. Employing multiple sequence alignments and phylogenic analysis, the mature Ppel-CHH peptide exhibited residues common to both CHH-type I and II peptides, and a high degree of identity to the type-I group, but little homology between Ppel-CHH and Ppel-MIH (a type II peptide). This sequence identification then allowed for the use of MIH antisera to further confirm the identity and existence of a MIH-ir 9kDa protein in all neural organs tested by Western blotting, and through immunohistochemistry, MIH-ir in the XO, optic nerve, neuronal cluster 17 of the supraesophageal ganglion, the ventral nerve cord, and cell cluster 22 of the thoracic ganglion. The presence of MIH protein within such a diversity of sites in the CNS, and external to the XOSG, raises new questions concerning the established mode of MIH action.
Details
- Title
- Cloning of the crustacean hyperglycemic hormone and evidence for molt-inhibiting hormone within the central nervous system of the blue crab Portunus pelagicus
- Authors
- Michael J Stewart (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringPraphaporn Stewart (Author) - Thammasat University, ThailandM Sroyraya (Author) - Mahidol University, ThailandN Soonklang (Author) - Thammasat University, ThailandScott F Cummins (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringP J Hanna (Author) - Deakin UniversityW Duan (Author) - Deakin UniversityP Sobhon (Author) - Mahidol University, Thailand
- Publication details
- Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology, Vol.164(2), pp.276-290
- Publisher
- Elsevier Inc.
- Date published
- 2013
- DOI
- 10.1016/j.cbpa.2012.10.029
- ISSN
- 1095-6433
- Copyright note
- Copyright © 2013. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
- Organisation Unit
- School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99450264102621
- Output Type
- Journal article
Metrics
197 File views/ downloads
983 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- International collaboration
- Web Of Science research areas
- Biochemistry & Molecular Biology
- Physiology
- Zoology
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites