Cloning of the cDNA and Localization of the Gene Encoding Human NRBP, a Ubiquitously Expressed, Multidomain Putative Adapter Protein

J D Hooper; E Baker; Steven Ogbourne; G R Sutherland; T M Antalis

doi:10.1006/geno.2000.6167

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Cloning of the cDNA and Localization of the Gene Encoding Human NRBP, a Ubiquitously Expressed, Multidomain Putative Adapter Protein

Journal article

Peer reviewed

Cloning of the cDNA and Localization of the Gene Encoding Human NRBP, a Ubiquitously Expressed, Multidomain Putative Adapter Protein

J D Hooper, E Baker, Steven Ogbourne, G R Sutherland and T M Antalis

Genomics, Vol.66(1), pp.113-118

2000

DOI: https://doi.org/10.1006/geno.2000.6167

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Abstract

Genetics

Information Systems

Adapter proteins modulate multiple signaling pathways by regulating the aggregation of other factors into signaling complexes. Here we have identified a novel human cDNA encoding NRBP, a multidomain putative adapter protein containing (i) two putative nuclear receptor binding motifs (LXXLL), (ii) a putative binding domain for Src homology-2 (SH2) domain containing proteins, (iii) a kinase-like domain, (iv) a bipartite nuclear localization signal, and (v) three sequences rich in glutamic acid, serine, proline, and threonine (PEST) residues. The NRBP mRNA transcript, of approximately 2.4 kb, was ubiquitously expressed in a wide range of normal human tissues and 15 human tumor cell lines. The NRBP cDNA is predicted to encode a polypeptide of 535 amino acids with a molecular mass of 59.8 kDa. Translation of NRBP mRNA in vitro reveals three translation products of 60, 51, and 43 kDa, suggesting that translation of NRBP may initiate at multiple sites. The NRBP gene was localized to human chromosome 2p23, near the location of the NCOA1 gene encoding the nuclear receptor coactivator, steroid receptor coactivator-1 (SRC-1). The features of NRBP predict a function as an adapter protein potentially linking signaling pathways involving nuclear receptors and SH2 domain containing proteins.

Details

Title: Cloning of the cDNA and Localization of the Gene Encoding Human NRBP, a Ubiquitously Expressed, Multidomain Putative Adapter Protein
Authors: J D Hooper (Author) - University of Queensland
E Baker (Author) - Women's and Children's Hospital, Adelaide
Steven Ogbourne (Author) - University of Queensland
G R Sutherland (Author) - Women's and Children's Hospital, Adelaide
T M Antalis (Author) - University of Queensland
Publication details: Genomics, Vol.66(1), pp.113-118
Publisher: Academic Press
Date published: 2000
DOI: 10.1006/geno.2000.6167
ISSN: 0888-7543
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99450163902621
Output Type: Journal article

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Collaboration types: Domestic collaboration
Web Of Science research areas: Biotechnology & Applied Microbiology; Genetics & Heredity

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