Cloning and characterization of the white and topaz eye color genes from the sheep blowfly Lucilia cuprina

Abigail Elizur; A T Vacek; A J Howells

doi:10.1007/BF02101889

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Cloning and characterization of the white and topaz eye color genes from the sheep blowfly Lucilia cuprina

Journal article

Peer reviewed

Cloning and characterization of the white and topaz eye color genes from the sheep blowfly Lucilia cuprina

Abigail Elizur, A T Vacek and A J Howells

Journal of Molecular Evolution, Vol.30(4), pp.347-358

1990

DOI: https://doi.org/10.1007/BF02101889

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Abstract

Biochemistry and Cell Biology

Evolutionary Biology

Genetics

cloning

eye colour genes

Lucilia cuprina

dispersed repeated sequences

polymorphism

Clones carrying the white and topaz eye color genes have been isolated from genomic DNA libraries of the blowfly Lucilia cuprina using cloned DNA from the homologous white and scarlet genes, respectively, of Drosophila melanogaster as probes. On the basis of hybridization studies using adjacent restriction fragments, homologous fragments were found to be colinear between the genes from the two species. The nucleotide sequence of a short region of the white gene of L. cuprina has been determined, and the homology to the corresponding region of D. melanogaster is 72%; at the derived amino acid level the homology is greater (84%) due to a marked difference in codon usage between the species. A major difference in genome organization between the two species is that whereas the DNA encompassing the D. melanogaster genes is free of repeated sequences, that encompassing their L. cuprina counterparts contains substantial amounts of repeated sequences. This suggests that the genome of L. cuprina is organized on the short period interspersion pattern. Repeated sequence DNA elements, which appear generally to be short (less than 1 kb) and which vary in repetitive frequency in the genome from greater than 104 copies to less than 102 copies, are found in at least two different locations in the clones carrying these genes. One type of repeat structure, found by sequencing, consists of tandemly repeating short sequences. Restriction site and restriction fragment length polymorphisms involving both the white and topaz gene regions are found within and between populations of L. cuprina.

Details

Title: Cloning and characterization of the white and topaz eye color genes from the sheep blowfly Lucilia cuprina
Authors: Abigail Elizur (Author) - University of the Sunshine Coast - Faculty of Science, Health and Education
A T Vacek (Author) - Australian National University
A J Howells (Author) - Australian National University
Publication details: Journal of Molecular Evolution, Vol.30(4), pp.347-358
Publisher: Springer New York LLC
Date published: 1990
DOI: 10.1007/BF02101889
ISSN: 0022-2844
Organisation Unit: University of the Sunshine Coast, Queensland; Centre for Bioinnovation
Language: English
Record Identifier: 99449265702621
Output Type: Journal article

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