Clonal groups of high-level gentamicin-resistant Enterococcus faecium isolated from municipal wastewater and clinical samples in Tehran, Iran

M Saifi; M R Pourshafie; M M Soltan Dallal; Mohammad Katouli

doi:10.1111/j.1472-765X.2009.02559.x

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Clonal groups of high-level gentamicin-resistant Enterococcus faecium isolated from municipal wastewater and clinical samples in Tehran, Iran

Journal article

Peer reviewed

Clonal groups of high-level gentamicin-resistant Enterococcus faecium isolated from municipal wastewater and clinical samples in Tehran, Iran

M Saifi, M R Pourshafie, M M Soltan Dallal and Mohammad Katouli

Letters in Applied Microbiology, Vol.49(2), pp.160-165

2009

DOI: https://doi.org/10.1111/j.1472-765X.2009.02559.x

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Abstract

Enterococcus faecium

HLGR

PFGE

typing

wastewater

Aims: Clonality among high-level gentamicin-resistant Enterococcus faecium (HLGR-EF) isolates obtained from clinical and sewage treatment plants (STP) were investigated using PhePlate system (PhP), ribotyping and pulsed-field gel electrophoresis (PFGE). Methods and Results: During 1 year study (September 2005–2006), a total of 106 HLGR-EF isolates were collected from clinical (n = 48) and STP (n = 58) samples in Tehran, Iran. Biochemical fingerprinting of these isolates using the PhP showed the presence of 21 PhP types (diversity index, Di = 0·97) among the clinical and 21 PhP types (Di = 0·91) among the STP isolates. Representative isolates of each PhP type (n = 42) were further characterized by the ribotyping method. Sixteen ribotypes were identified among the isolates with five types shared between the clinical and STP isolates. PFGE recognized 24 clonal types among these isolates with three pulsotypes shared between the clinical and STP isolates. Combination of the two techniques (PFGE and ribotyping) resulted in 24 (Di = 0·96) and 16 (Di = 0·93) types among the strains isolated from clinical and STP samples, respectively. Conclusions: We concluded that the combination of PhP typing, ribotyping and PFGE could be extremely discriminatory when examining HLGR-EF isolates. Significance and Impact of the Study: The emergence of highly diverse HLGR-EF population in Iran is of serious concern especially because of their multi-resistances.

Details

Title: Clonal groups of high-level gentamicin-resistant Enterococcus faecium isolated from municipal wastewater and clinical samples in Tehran, Iran
Authors: M Saifi (Author) - Pasteur Institute of Iran, Iran
M R Pourshafie (Author) - Pasteur Institute of Iran, Iran
M M Soltan Dallal (Author) - Tehran Medical University, Iran
Mohammad Katouli (Author) - University of the Sunshine Coast - Faculty of Science, Health and Education
Publication details: Letters in Applied Microbiology, Vol.49(2), pp.160-165
Publisher: Wiley-Blackwell Publishing Ltd.
Date published: 2009
DOI: 10.1111/j.1472-765X.2009.02559.x
ISSN: 0266-8254
Organisation Unit: University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99449701402621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Biotechnology & Applied Microbiology; Microbiology

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