Chromosome set manipulations and hybridization experiments in gilthead seabream (Sparus aurata). I. Induction of gynogenesis and intergeneric hybridization using males of the red sea bream, Pagrus major

G Gorshkova; S Gorshkov; A Hadani; H Gordin; Wayne R Knibb

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Chromosome set manipulations and hybridization experiments in gilthead seabream (Sparus aurata). I. Induction of gynogenesis and intergeneric hybridization using males of the red sea bream, Pagrus major

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Chromosome set manipulations and hybridization experiments in gilthead seabream (Sparus aurata). I. Induction of gynogenesis and intergeneric hybridization using males of the red sea bream, Pagrus major

G Gorshkova, S Gorshkov, A Hadani, H Gordin and Wayne R Knibb

Israeli Journal of Aquaculture: Bamidgeh, Vol.50(3), pp.99-110

1998

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Abstract

Fisheries Sciences

Sparus aurata

hybridization

Pagrus major

The parameters for the production of triploid and gynogenetic progenies of the gilthead seabream, Sparus aurata, have been established. To induce diploidization of the maternal genome the optimal heat shock temperature used was 36.5-97.gC. Time after fertilization for shock treatment and duration of shock were 3 and 2.5 min, respectively. Triploidization efficiency in embryos was positively associated with shock temperature, reaching a maximum of 1OO/" at 37'C- Gynogenetic diploid progenies ol S. auratawere produced using the same conditiongestablished for triploidization, except that fertilization was carried out with UV-inactivated heterologous sperm of red seabream, Pagrus maior. The efficiency of the sperm inactivation was estimated to be 100%' Diploid and triploid hybrids between S. auratafemales and P. majormales were produced. The chromosome "markers" useful in interspecific hybridization experiments are discussed.

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Title: Chromosome set manipulations and hybridization experiments in gilthead seabream (Sparus aurata). I. Induction of gynogenesis and intergeneric hybridization using males of the red sea bream, Pagrus major
Authors: G Gorshkova (Author) - National Center for Mariculture, Israel
S Gorshkov (Author) - National Center for Mariculture, Israel
A Hadani (Author) - National Center for Mariculture, Israel
H Gordin (Author) - National Center for Mariculture, Israel
Wayne R Knibb (Author) - National Center for Mariculture, Israel
Publication details: Israeli Journal of Aquaculture: Bamidgeh, Vol.50(3), pp.99-110
Publisher: Israeli Journal of Aquaculture - Bamidgeh
Date published: 1998
ISSN: 0792-156X
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland
Language: English
Record Identifier: 99450301302621
Output Type: Journal article

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