Journal article
Chlamydophila pneumoniae HflX belongs to an uncharacterized family of conserved GTPases and associates with the Escherichia coli 50S large ribosomal subunit
Microbiology, Vol.154(11), pp.3537-3546
2008
Abstract
Predicted members of the HflX subfamily of phosphate-binding-loop guanosine triphosphatases (GTPases) are widely distributed in the bacterial kingdom but remain virtually uncharacterized. In an attempt to understand mechanisms used for regulation of growth and development in the chlamydiae, obligate intracellular and developmentally complex bacteria, we have begun investigations into chlamydial GTPases; we report here what appears to be the first analysis of a HflX family GTPase using a predicted homologue from Chlamydophila pneumoniae. In agreement with phylogenetic predictions for members of this GTPase family, purified recombinant Cp. pneumoniae HflX was specific for guanine nucleotides and exhibited a slow intrinsic GTPase activity when incubated with [γ-32P]GTP. Using HflX-specific monoclonal antibodies, HflX could be detected by Western blotting and high-resolution confocal microscopy throughout the vegetative growth cycle of Cp. pneumoniae and, at early time points, appeared to partly localize to the membrane. Ectopic expression of Cp. pneumoniae HflX in Escherichia coli revealed cosedimentation of HflX with the E. coli 50S large ribosomal subunit. The results of this work open up some intriguing possibilities for the role of GTPases belonging to this previously uncharacterized family of bacterial GTPases. Ribosome association is a feature shared by other important conserved GTPase families and more detailed investigations will be required to delineate the role of HflX in bacterial ribosome function. © 2008 SGM.
Details
- Title
- Chlamydophila pneumoniae HflX belongs to an uncharacterized family of conserved GTPases and associates with the Escherichia coli 50S large ribosomal subunit
- Authors
- Adam Polkinghorne (Author) - University of Zurich, SwitzerlandU Ziegler (Author) - University of Zurich, SwitzerlandY González-Hernández (Author) - ETH Honggerberg, SwitzerlandA Pospischil (Author) - University of Zurich, SwitzerlandPeter Timms (Author) - Queensland University of TechnologyL Vaughan (Author) - Queensland University of Technology
- Publication details
- Microbiology, Vol.154(11), pp.3537-3546
- Publisher
- Society for General Microbiology
- Date published
- 2008
- DOI
- 10.1099/mic.0.2008/022137-0
- ISSN
- 1350-0872
- Organisation Unit
- University of the Sunshine Coast, Queensland; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99448994802621
- Output Type
- Journal article
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