Chlamydial clinical isolates show subtle differences in persistence phenotypes and growth in vitro

Mark Thomas; Amba Lawrence; Samuel Kroon; Lenka A Vodstrcil; Samuel Phillips; Jane S Hocking; Peter Timms; Wilhelmina M Huston

doi:10.1099/acmi.0.000204

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Chlamydial clinical isolates show subtle differences in persistence phenotypes and growth in vitro

Journal article

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Chlamydial clinical isolates show subtle differences in persistence phenotypes and growth in vitro

Mark Thomas, Amba Lawrence, Samuel Kroon, Lenka A Vodstrcil, Samuel Phillips, Jane S Hocking, Peter Timms and Wilhelmina M Huston

Access Microbiology, Vol.3(3), pp.1-12

2021

DOI: https://doi.org/10.1099/acmi.0.000204

PMCID: PMC8209716

PMID: 34151159

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Abstract

Chlamydia trachomatis

persistence

clinical isolates

iron

penicilin

Urogenital Chlamydia trachomatis infection is the most common sexually transmitted bacterial infection throughout the world. While progress has been made to better understand how type strains develop and respond to environmental stress in vitro, very few studies have examined how clinical isolates behave under similar conditions. Here, we examined the development and persistence phenotypes of several clinical isolates, to determine how similar they are to each other, and the type strain C. trachomatis D/UW-3/Cx. The type strain was shown to produce infectious progeny at a higher magnitude than each of the clinical isolates, in each of the six tested cell lines. All chlamydial strains produced the highest number of infectious progeny at 44 h post-infection in the McCoy B murine fibroblast cell line, yet showed higher levels of infectivity in the MCF-7 human epithelial cell line. The clinical isolates were shown to be more susceptible than the type strain to the effects of penicillin and iron deprivation persistence models in the MCF-7 cell line. While subtle differences between clinical isolates were observed throughout the experiments conducted, no significant differences were identified. This study reinforces the importance of examining clinical isolates when trying to relate in vitro data to clinical outcomes, as well as the importance of considering the adaptations many type strains have to being cultured in vitro.

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Title: Chlamydial clinical isolates show subtle differences in persistence phenotypes and growth in vitro
Authors: Mark Thomas (Author) - University of Technology Sydney
Amba Lawrence (Author) - Queensland University of Technology
Samuel Kroon (Author) - University of Technology Sydney
Lenka A Vodstrcil (Author) - Monash University
Samuel Phillips (Author) - Murdoch Childrens Research Institute
Jane S Hocking (Author) - The University of Melbourne
Peter Timms (Author) - University of the Sunshine Coast, Queensland, GeneCology Research Centre - Legacy
Wilhelmina M Huston (Corresponding Author) - University of Technology Sydney
Publication details: Access Microbiology, Vol.3(3), pp.1-12
Publisher: The Microbiology Society
Date published: 2021
DOI: 10.1099/acmi.0.000204
PMID: 34151159; PMC8209716
Copyright note: This is an open-access article distributed under the terms of the Creative Commons Attribution License
Organisation Unit: University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99511206502621
Output Type: Journal article

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