Chlamydia Serine Protease Inhibitor, targeting HtrA, as a New Treatment for Koala Chlamydia infection

Amba Lawrence; Tamieka A Fraser; Amber Gillett; Joel D A Tyndall; Peter Timms; Adam Polkinghorne; Wilhelmina M Huston

doi:10.1038/srep31466

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Chlamydia Serine Protease Inhibitor, targeting HtrA, as a New Treatment for Koala Chlamydia infection

Journal article

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Peer reviewed

Chlamydia Serine Protease Inhibitor, targeting HtrA, as a New Treatment for Koala Chlamydia infection

Amba Lawrence, Tamieka A Fraser, Amber Gillett, Joel D A Tyndall, Peter Timms, Adam Polkinghorne and Wilhelmina M Huston

Scientific Reports, Vol.6(31466)

2016

DOI: https://doi.org/10.1038/srep31466

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Abstract

antimicrobials

applied microbiology

The koala, an iconic marsupial native to Australia, is a threatened species in many parts of the country. One major factor in the decline is disease caused by infection with Chlamydia. Current therapeutic strategies to treat chlamydiosis in the koala are limited. This study examines the effectiveness of an inhibitor, JO146, which targets the HtrA serine protease for treatment of C. pecorum and C. pneumoniae in vitro and ex vivo with the aim of developing a novel therapeutic for koala Chlamydia infections. Clinical isolates from koalas were examined for their susceptibility to JO146. In vitro studies demonstrated that treatment with JO146 during the mid-replicative phase of C. pecorum or C. pneumoniae infections resulted in a significant loss of infectious progeny. Ex vivo primary koala tissue cultures were used to demonstrate the efficacy of JO146 and the non-toxic nature of this compound on peripheral blood mononuclear cells and primary cell lines established from koala tissues collected at necropsy. Our results suggest that inhibition of the serine protease HtrA could be a novel treatment strategy for chlamydiosis in koalas.

Details

Title: Chlamydia Serine Protease Inhibitor, targeting HtrA, as a New Treatment for Koala Chlamydia infection
Authors: Amba Lawrence (Author) - Queensland University of Technology
Tamieka A Fraser (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Amber Gillett (Author) - Australia Zoo Wildlife Hospital
Joel D A Tyndall (Author) - University of Otago, New Zealand
Peter Timms (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Adam Polkinghorne (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Wilhelmina M Huston (Author) - Queensland University of Technology
Publication details: Scientific Reports, Vol.6(31466); 12
Publisher: Nature Publishing Group
Date published: 2016
DOI: 10.1038/srep31466
ISSN: 2045-2322
Copyright note: Copyright © 2016 The Authors. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; Centre for Bioinnovation
Language: English
Record Identifier: 99451051302621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Microbiology

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