Journal article
Chiral capillary electrophoresis with cationic pyrrolidinium-β- cyclodextrin derivatives as chiral selectors
Journal of Separation Science, Vol.33(12), pp.1797-1805
2010
Abstract
New single-isomer, cationic β-cyclodextrins, including mono-6-deoxy-6-pyrrolidine-β-cyclodextrin chloride (pyCDCl), mono-6-deoxy-6-(N-methyl-pyrrolidine)-β-cyclodextrin chloride (N-CH <lnf>3</inf>-pyCDCl), mono-6-deoxy-6-(N-(2-hydroxyethyl)-pyrrolidine)-β- cyclodextrin chloride (NEtOH-pyCDCl), mono-6-deoxy-6-(2-hydroxymethyl- pyrrolidine)-β-cyclodextrin chloride (2-MeOH-pyCDCl) were synthesized and used as chiral selectors in capillary electrophoresis for the enantioseparation of carboxylic and hydroxycarboxylic acids and dansyl amino acids. The unsubstituted pyCDCl exhibited the greatest resolving ability. Most analytes were resolved over a wide range of pH from 6.0 to 9.0 with this chiral selector. In general, increasing pH led to a decrease in resolution. The effective mobilities of all the analytes were found to decrease with increasing CD concentration. The optimal concentration for most carboxylic acids and dansyl amino acid was in the range 5-7.5mM and >15mM for hydroxycarboxylic acids. 1H NMR experiments provided direct evidence of inclusion in the CD cavity. ©2010 Wiley-VCH Verlag GmbH & Co. KGaA.
Details
- Title
- Chiral capillary electrophoresis with cationic pyrrolidinium-β- cyclodextrin derivatives as chiral selectors
- Authors
- Y Xiao (Author) - Nanyang Technological University, SingaporeY Wang (Author) - Nanyang Technological University, SingaporeT T Ong (Author) - Nanyang Technological University, SingaporeL Ge (Author) - Nanyang Technological University, SingaporeS N Tan (Author) - Nanyang Technological University, SingaporeDavid James Young (Author) - Griffith UniversityT T Y Tan (Author) - Nanyang University, SingaporeS C Ng (Author) - Nanyang University, Singapore
- Publication details
- Journal of Separation Science, Vol.33(12), pp.1797-1805
- Publisher
- Wiley - V C H Verlag GmbH & Co. KGaA
- DOI
- 10.1002/jssc.200900732
- ISSN
- 1615-9306
- Organisation Unit
- University of the Sunshine Coast, Queensland
- Language
- English
- Record Identifier
- 99449819602621
- Output Type
- Journal article