Characterization of type 1 collagen from the skin of blue grenadier (Marcruronus novaezelandiae)

J A M Ramshaw; J A Werkmeister; H A Bremner

doi:10.1016/0003-9861(88)90056-2

Back

Characterization of type 1 collagen from the skin of blue grenadier (Marcruronus novaezelandiae)

Journal article

Peer reviewed

Characterization of type 1 collagen from the skin of blue grenadier (Marcruronus novaezelandiae)

J A M Ramshaw, J A Werkmeister and H A Bremner

Archives of Biochemistry and Biophysics, Vol.267(2), pp.497-502

1988

DOI: https://doi.org/10.1016/0003-9861(88)90056-2

Files and links (1)

url

https://doi.org/10.1016/0003-9861(88)90056-2View

Published Version

Abstract

Biochemistry and Cell Biology

The skin collagen of a fish, blue grenadier (Macruronus novaezelandiae), has been purified and characterized. The fish skin was readily soluble in dilute acetic acid, with no pepsin treatment needed. The collagen was purified by salt precipitation. Skin samples from fish of various ages showed that even in the oldest sample, more than 8 years of age, the collagen was still readily acid soluble. The purified collagen had a melting temperature of 22 °C; the shrinkage temperature for the skin was 48 °C. Its tissue distribution, examined by immunohistology, and its chemical properties indicated a close homology to mammalian type I collagen. However, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) showed that three distinct α-chains were present. These were purified by ion-exchange chromatography on CM-cellulose and by gel permeation chromatography on Superose 6. The three purified α-chain fractions were examined by amino acid analysis and by SDS-PAGE of their cyanogen bromide fragments. These data indicated that the additional chain was genetically distinct, and most closely related to the α1-chain, from which it was poorly resolved on SDS-PAGE.

Details

Title: Characterization of type 1 collagen from the skin of blue grenadier (Marcruronus novaezelandiae)
Authors: J A M Ramshaw (Author) - CSIRO Division of Protein Chemistry
J A Werkmeister (Author) - CSIRO Division of Protein Chemistry
H A Bremner (Author) - CSIRO Division of Fisheries Research
Publication details: Archives of Biochemistry and Biophysics, Vol.267(2), pp.497-502
Publisher: Academic Press
Date published: 1988
DOI: 10.1016/0003-9861(88)90056-2
ISSN: 0003-9861
Organisation Unit: University of the Sunshine Coast, Queensland
Language: English
Record Identifier: 99450275002621
Output Type: Journal article

Metrics

4 File views/ downloads

852 Record Views

27 Times Cited - Web of Science

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Web Of Science research areas: Biochemistry & Molecular Biology; Biophysics