Characterization of tilapia (Oreochromis niloticus) gonadotropins by modeling and immunoneutralization

Joseph Aizen; Noga Kowalsman; Y Niv Masha; Berta Levavi-Sivan

doi:10.1016/j.ygcen.2014.05.028

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Characterization of tilapia (Oreochromis niloticus) gonadotropins by modeling and immunoneutralization

Journal article

Peer reviewed

Characterization of tilapia (Oreochromis niloticus) gonadotropins by modeling and immunoneutralization

Joseph Aizen, Noga Kowalsman, Y Niv Masha and Berta Levavi-Sivan

General and Comparative Endocrinology, Vol.207, pp.28-33

2014

DOI: https://doi.org/10.1016/j.ygcen.2014.05.028

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Abstract

Zoology

Physiology

Veterinary Sciences

11-ketotestosterone

FSH

GnRH

Gonadotropin receptor

In fish, both follicle-stimulating hormone (FSH) and luteinizing hormone (LH) play important roles in reproduction. Here we explored the structure and differential specificity of tilapia (t) gonadotropins (GTHs) to delineate their physiological relevance and the nature of their regulation. We generated structural models of tGTHs and GTH receptors (R) that enabled us to better understand the hormone-receptor interacting region. In tilapia, FSH release is under the control of the hypothalamic decapeptide GnRH, an effect that was abolished by specific bioneutralizing antisera [anti-recombinant (r) tFSHβ]. These antisera also reduced the basal secretion and delayed GnRH-stimulated production of 11-ketotestosterone (11KT), and dramatically reduced LH levels. Immunoneutralization of tLH using anti-rtLHβ significantly reduced its GnRH-stimulated levels. Basal 11KT and FSH levels were also reduced. Taken together, these results suggest a feedback mechanism between FSH and LH release in tilapia.

Details

Title: Characterization of tilapia (Oreochromis niloticus) gonadotropins by modeling and immunoneutralization
Authors: Joseph Aizen (Author) - Hebrew University of Jerusalem, Israel
Noga Kowalsman (Author) - Hebrew University of Jerusalem, Israel
Y Niv Masha (Author) - Hebrew University of Jerusalem, Israel
Berta Levavi-Sivan (Author) - Hebrew University of Jerusalem, Israel
Publication details: General and Comparative Endocrinology, Vol.207, pp.28-33
Publisher: Academic Press
Date published: 2014
DOI: 10.1016/j.ygcen.2014.05.028
ISSN: 0016-6480
Organisation Unit: University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy; Centre for Bioinnovation
Language: English
Record Identifier: 99448734902621
Output Type: Journal article

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