Characterization of the fecal microbiota of pigs before and after inoculation with "Brachyspira hampsonii"

M O Costa; Bonnie L Chaban; J C S Harding; J E Hill

doi:10.1371/journal.pone.0106399

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Characterization of the fecal microbiota of pigs before and after inoculation with "Brachyspira hampsonii"

Journal article

Open access

Peer reviewed

Characterization of the fecal microbiota of pigs before and after inoculation with "Brachyspira hampsonii"

M O Costa, Bonnie L Chaban, J C S Harding and J E Hill

PLoS One, Vol.9(8), e106399

2014

DOI: https://doi.org/10.1371/journal.pone.0106399

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Abstract

Brachyspira hampsonii causes disease indistinguishable from swine dysentery, and the structure of the intestinal microbiome likely plays a role in determining susceptibility of individual pigs to infection and development of clinical disease. The objectives of the current study were to determine if the pre-inoculation fecal microbiota differed between inoculated pigs that did (INOC MH) or did not (INOC non-MH) develop mucohaemorrhagic diarrhea following challenge with "B. hampsonii", and to quantify changes in the structure of the microbiome following development of clinical disease. Fecal microbiota profiles were generated based on amplification and sequencing of the cpn60 universal target sequence from 89 samples from 18 pigs collected at -8, -5, -3 and 0 days post-inoculation, and at termination. No significant differences in richness, diversity or taxonomic composition distinguished the pre-inoculation microbiomes of INOC MH and INOC non-MH pigs. However, the development of bloody diarrhea in inoculated pigs was associated with perturbation of the microbiota relative to INOC non-MH or sham-inoculated control pigs. Specifically, the fecal microbiota of INOC MH pigs was less dense (fewer total 16S rRNA copies per gram of feces), and had a lower Bacteroidetes:Firmicutes ratio. Further investigation of the potential long-term effects of Brachyspira disease on intestinal health and performance is warranted. © 2014 Costa et al.

Details

Title: Characterization of the fecal microbiota of pigs before and after inoculation with "Brachyspira hampsonii"
Authors: M O Costa (Author) - University of Saskatchewan, Canada
Bonnie L Chaban (Author) - University of Saskatchewan, Canada
J C S Harding (Author) - University of Saskatchewan, Canada
J E Hill (Author) - University of Saskatchewan, Canada
Publication details: PLoS One, Vol.9(8), e106399; 6
Publisher: Public Library of Science
Date published: 2014
DOI: 10.1371/journal.pone.0106399
ISSN: 1932-6203
Copyright note: Copyright © 2014 Costa et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Organisation Unit: University of the Sunshine Coast, Queensland; Thompson Institute; Centre for Bioinnovation
Language: English
Record Identifier: 99450572102621
Output Type: Journal article

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