Characterization of red pigment concentrating hormone (RPCH) in the female mud crab (Scylla olivacea) and the effect of 5-HT on its expression

N Kornthong; C Chotwiwatthanakun; P Chansela; Y Tinikul; Scott F Cummins; P J Hanna; P Sobhon

doi:10.1016/j.ygcen.2013.01.011

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Characterization of red pigment concentrating hormone (RPCH) in the female mud crab (Scylla olivacea) and the effect of 5-HT on its expression

Journal article

Open access

Peer reviewed

Characterization of red pigment concentrating hormone (RPCH) in the female mud crab (Scylla olivacea) and the effect of 5-HT on its expression

N Kornthong, C Chotwiwatthanakun, P Chansela, Y Tinikul, Scott F Cummins, P J Hanna and P Sobhon

General and Comparative Endocrinology, Vol.185, pp.28-36

2013

DOI: https://doi.org/10.1016/j.ygcen.2013.01.011

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Abstract

red pigment concentrating hormone (RPCH)

5-HT

Scylla olivacea

mud crab

distribution

Red pigment concentrating hormone (RPCH) is a member of the chromatophorotropic hormones and, in crustaceans, it is synthesized in the eyestalk. We have isolated a full length cDNA for a RPCH preprohormone gene (Scyol-RPCH) from the eyestalks of female mud crabs, Scylla olivacea. The open reading frame consists of 642 nucleotides, and encodes a deduced 108 amino acid precursor protein, which includes a signal peptide, the RPCH (pQLNFSPGWamide), and an associated peptide. We show that the mud crab RPCH peptide exhibits 100% identity with 15 other decapods. Expression of Scyol-RPCH within adult mud crab takes place in the eyestalk, brain, and ventral nerve cord, comprising subesophageal ganglion, thoracic ganglion, and abdominal ganglion. In situ hybridization demonstrates specific expression within neuronal clusters 1, 2, 3, and 4 of the eyestalk X-organ, clusters 6, 8, 9, 10, and 17 of the brain, and in neuronal clusters of the ventral nerve cord. We found that administration of 5-HT up-regulates RPCH gene expression in the eyestalk, suggesting that RPCH may play a role as a downstream hormone of 5-HT.

Details

Title: Characterization of red pigment concentrating hormone (RPCH) in the female mud crab (Scylla olivacea) and the effect of 5-HT on its expression
Authors: N Kornthong (Author) - Mahidol University, Thailand
C Chotwiwatthanakun (Author) - Mahidol University, Thailand
P Chansela (Author) - Mahidol University, Thailand
Y Tinikul (Author) - Mahidol University, Thailand
Scott F Cummins (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
P J Hanna (Author) - Deakin University
P Sobhon (Author) - Mahidol University, Thailand
Publication details: General and Comparative Endocrinology, Vol.185, pp.28-36
Publisher: Academic Press
Date published: 2013
DOI: 10.1016/j.ygcen.2013.01.011
ISSN: 0016-6480; 0016-6480
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99450024602621
Output Type: Journal article
Research Statement: false

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Endocrinology & Metabolism; Zoology

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