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Characterization of phosphorus compounds in soils by deep ultraviolet (DUV) Raman microspectroscopy
Journal article   Open access   Peer reviewed

Characterization of phosphorus compounds in soils by deep ultraviolet (DUV) Raman microspectroscopy

C Vogel, M Ramsteiner, Ryo Sekine, A Doolette and C Adam
Journal of Raman Spectroscopy, Vol.48(6), pp.867-871
2017
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Characterization of phosphorus compounds in soils by deep ultraviolet (DUV) Raman microspectroscopy1,016.41 kBDownloadView
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https://doi.org/10.1002/jrs.5115View
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Abstract

deep ultraviolet (DUV) Raman microspectroscopy phosphorus speciation soil
Deep ultraviolet Raman microspectroscopy was successfully investigated as a new approach to analyze the chemical state of phosphorus compounds directly in soil. We demonstrate that ultraviolet excitation has the advantage to avoid the interference with the strong fluorescence, which occurs in the visible spectral range caused by organic matter in soils. Furthermore, the spatial resolution of <1 μm2 enables the detection of very small phosphorus particles. For some organic phosphorus compounds (β-glycerophosphate, aminomethylphosphonic acid), sample cooling to -100 °C is found to strongly reduce the rate of degradation induced by the illumination with the ultraviolet excitation light. However, phytic acid and adenosine monophosphate degraded even with cooling. Our results reveal the capability of deep ultraviolet Raman microspectroscopy as a high-resolution benchtop imaging technique for the analysis of local interactions between soil compounds with the potential to become an analytical key to improve the understanding of transformation mechanisms of phosphates as well as other mineral phases in soils.

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