Journal article
Characterization of in vitro chlamydia muridarum persistence and utilization in an in vivo mouse model of chlamydia vaccine
American Journal of Reproductive Immunology, Vol.69(5), pp.475-485
2013
Abstract
Problem: Chlamydia trachomatis genital tract infections are easily treated with antibiotics; however, the majority of infections are asymptomatic and therefore untreated, highlighting the need for a vaccine. Because most infections are asymptomatic, vaccination could potentially be administered to individuals who may have an acute infection at that time. In such individuals, the effect of vaccination on the existing infection is unknown; however, one potential outcome could be the development of a persistent infection. In vitro chlamydial persistence has been well characterized in various strains; however, there have been no reported studies in C.muridarum. Method of Study: We performed ultrastructural characterization and transcriptome analysis of selected genes. We then used the transcriptional profiles of the selected genes to examine whether intranasal immunization of mice during an active genital infection would induce persistence in the upper reproductive tract of female mice. Results and Conclusions: We found that persistence developed in the oviducts of mice as a result of immunization. This is a significant finding, not only because it is the first time that C.muridarum persistence has been characterized in vitro, but also due to the fact that there is a minimal characterization of in vivo persistence of any chlamydial species. This highlights the importance of the timing of vaccination in individuals.
Details
- Title
- Characterization of in vitro chlamydia muridarum persistence and utilization in an in vivo mouse model of chlamydia vaccine
- Authors
- A J Carey (Author) - Queensland University of TechnologyW M Huston (Author) - Queensland University of TechnologyK A Cunningham (Author) - Queensland University of TechnologyL M Hafner (Author) - Queensland University of TechnologyPeter Timms (Author) - Queensland University of TechnologyK W Beagley (Author) - Queensland University of Technology
- Publication details
- American Journal of Reproductive Immunology, Vol.69(5), pp.475-485
- Publisher
- Wiley-Blackwell Publishing Inc.
- Date published
- 2013
- DOI
- 10.1111/aji.12093
- ISSN
- 1046-7408
- Copyright note
- Copyright © 2013 Wiley-Blackwell Publishing Inc. This is the accepted version of the following article: Carey AJ, Huston WM, Cunningham KA, Hafner LM, Timms P, Beagley KW. Characterization of in vitro Chlamydia muridarum persistence and utilization in an in vivo mouse model of Chlamydia vaccine. Am J Reprod Immunol 2013; 69: 475-485, which has been published in final form at http://dx.doi.org/10.1111/aji.12093
- Organisation Unit
- University of the Sunshine Coast, Queensland; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99449058802621
- Output Type
- Journal article
Metrics
96 File views/ downloads
704 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Web Of Science research areas
- Immunology
- Reproductive Biology
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites