Characterization of an abalone gonadotropin-releasing hormone and its effect on ovarian cell proliferation

Parinyaporn Nuurai; Scott F Cummins; Natasha A Botwright; Prasert Sobhon

doi:10.1016/j.aquaculture.2015.07.008

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Characterization of an abalone gonadotropin-releasing hormone and its effect on ovarian cell proliferation

Journal article

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Characterization of an abalone gonadotropin-releasing hormone and its effect on ovarian cell proliferation

Parinyaporn Nuurai, Scott F Cummins, Natasha A Botwright and Prasert Sobhon

Aquaculture, Vol.450, pp.116-122

2016

DOI: https://doi.org/10.1016/j.aquaculture.2015.07.008

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Abstract

gonadotropin-releasing hormone

haliotis asinina

haliotis laevigata

oogonia

oocyte

proliferation

BrdU assay

In various animal groups, both vertebrate and invertebrate, it has been reported that gonadotropin-releasing hormone (GnRH) is an important regulator of reproductive function. Knowledge of the molecular identity of a GnRH and its function in the abalone is unknown. In this study, we have characterized the abalone GnRH genes and their putative precursor sequences from the neural ganglia of sexually mature female Haliotis asinina and Haliotis laevigata. The abalone GnRH genes demonstrate high similarity with other gastropods within the proposed bioactive GnRH peptide regions (H. asinina: QNYHFSNGWYPG; H. laevigata: QNYHFSNGWHA) but less in the GnRH-associated peptide region. An antibody generated against HasGnRH showed specific expression of the peptide within the cerebral and pleuropedal ganglia. HasGnRH was localized in type-1 neurosecretory cells that are widely distributed within the ventral and dorsal parts of the cerebral ganglion and also observed in nerve fiber bundles extending into the dorsal part of the ganglion. In the pleuropedal ganglion, HasGnRH immunoreactive cells were distributed in both ventral and dorsal horns. Nerve fibers containing HasGnRH immunoreactivity were also detected primarily within the ventral region of ventral horns of the ganglion. Synthetic HasGnRH and buserelin stimulated oogonia and oocyte proliferation in one-year-old female H. asinina as determined by in vivo and in vitro BrdU ovarian cell proliferation. These findings provide important knowledge into the molecular identity of the abalone GnRH and its function in stimulating oocyte proliferation that helps us to further understand the role of this peptide in molluscs for which may be applied to aquaculture of this species.

Details

Title: Characterization of an abalone gonadotropin-releasing hormone and its effect on ovarian cell proliferation
Authors: Parinyaporn Nuurai (Author) - Burapha University, Thailand
Scott F Cummins (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Natasha A Botwright (Author) - CSIRO
Prasert Sobhon (Author) - Mahidol University, Thailand
Publication details: Aquaculture, Vol.450, pp.116-122
Publisher: Elsevier BV
Date published: 2016
DOI: 10.1016/j.aquaculture.2015.07.008
ISSN: 0044-8486
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99450077002621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Fisheries; Marine & Freshwater Biology

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