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Journal article
Characterization of Sarcoptes scabiei Tropomyosin and Paramyosin: Immunoreactive Allergens in Scabies
American Journal of Tropical Medicine and Hygiene, Vol.97(3), pp.851-860
2017
Appears in UniSC Supported Open Access Outputs
Abstract
Scabies is a human skin disease due to the burrowing ectoparasite Sarcoptes scabiei var. hominis resulting in intense itching and inflammation and manifesting as a skin allergy. Because of insufficient mite material and lack of in vitro propagation system for antigen preparation, scabies is a challenging disease to develop serological diagnostics. For allergen characterization, full-length S. scabiei tropomyosin (Sar s 10) was cloned, expressed in pET-15b, and assessed for reactivity with IgE antibodies from human sera. IgE binding was observed to Sar s 10 with sera collected from subjects with ordinary scabies, house dust mite (HDM)-positive and naive subjects and a diagnostic sensitivity of < 30% was observed. S. scabiei paramyosin (Sar s 11) was cloned, and expressed in pET-28a in three overlapping fragments designated Sspara1, Sspara2, and Sspara3. IgE and IgG binding was observed to Sspara2 and Sspara3 antigens with sera collected from ordinary scabies, and HDM-positive subjects, but no binding was observed with sera collected from naive subjects. Sspara2 displayed excellent diagnostic potential with 98% sensitivity and 90% specificity observed for IgE binding and 70% sensitivity for IgG. In contrast, the diagnostic sensitivity of Sspara3 was 84% for IgE binding and 40% for IgG binding. In combination, Sspara2 and Sspara3 provided an IgE sensitivity of 94%. This study shows that IgE binding to Sspara2 and Sspara3 is a highly sensitive method for diagnosis of scabies infestation in clinical practice. The developed enzyme-linked immunosorbent assay helps direct future development of a specific diagnostic tool for scabies.
Details
- Title
- Characterization of Sarcoptes scabiei Tropomyosin and Paramyosin: Immunoreactive Allergens in Scabies
- Authors
- Shumaila Naz (Author) - Pir Mehr Ali Shah Arid Agriculture University Rawalpindi, PakistanMarion Desclozeaux (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringKate E Mounsey (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringFarhana Riaz Chaudhry (Author) - Pir Mehr Ali Shah Arid Agriculture University Rawalpindi, PakistanShelley F Walton (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
- Publication details
- American Journal of Tropical Medicine and Hygiene, Vol.97(3), pp.851-860
- Publisher
- American Society of Tropical Medicine and Hygiene
- Date published
- 2017
- DOI
- 10.4269/ajtmh.16-0976
- ISSN
- 0002-9637
- Copyright note
- Copyright © 2017 by The American Society of Tropical Medicine and Hygiene. Reproduced here in accordance with the publisher's copyright policy.
- Organisation Unit
- School of Health - Biomedicine; School of Education and Tertiary Access; School of Health and Sport Sciences - Legacy; School of Health and Behavioural Sciences - Legacy
- Language
- English
- Record Identifier
- 99450446102621
- Output Type
- Journal article
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- Domestic collaboration
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- Public, Environmental & Occupational Health
- Tropical Medicine
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