Characterization of Microsatellite Primers in the Endangered Orchid Phaius australis and Cross-Amplification to P. bernaysii (Orchidaceae)

Laura Simmons; Robert W Lamont; Alison Shapcott

doi:10.3732/apps.1700085

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Characterization of Microsatellite Primers in the Endangered Orchid Phaius australis and Cross-Amplification to P. bernaysii (Orchidaceae)

Journal article

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Characterization of Microsatellite Primers in the Endangered Orchid Phaius australis and Cross-Amplification to P. bernaysii (Orchidaceae)

Laura Simmons, Robert W Lamont and Alison Shapcott

Applications in Plant Sciences, Vol.5(12), pp.1-3

2017

DOI: https://doi.org/10.3732/apps.1700085

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Abstract

Australia

conservation

microsatellite primers

orchid

Orchidaceae

Phaius australis

population genetics

Premise of the study: The swamp orchid, Phaius australis (Orchidaceae), is nationally endangered due to illegal collection and habitat loss and fragmentation, resulting in a disjunct distribution in spring and coastal wetland ecotones along Australia's east coast. Polymorphic microsatellite markers were developed to study genetic diversity and population structure for conservation and restoration purposes. Methods and Results: Illumina HiSeq high-throughput sequencing was used to develop 15 nuclear microsatellite markers, including 10 polymorphic markers for P. australis. Polymorphism at each marker was evaluated using 90 individuals from four natural populations. The number of alleles per locus ranged from one to three, and the observed and expected heterozygosity varied from 0.036 to 0.944 and from 0.035 to 0.611, respectively. These markers transferred successfully to congener P. bernaysii. Conclusions: The microsatellite markers will be useful for revealing levels of genetic diversity and gene flow for P. australis and may inform future conservation efforts. .

Details

Title: Characterization of Microsatellite Primers in the Endangered Orchid Phaius australis and Cross-Amplification to P. bernaysii (Orchidaceae)
Authors: Laura Simmons (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Robert W Lamont (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Alison Shapcott (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Publication details: Applications in Plant Sciences, Vol.5(12), pp.1-3
Publisher: Botanical Society of America, Inc.
Date published: 2017
DOI: 10.3732/apps.1700085
ISSN: 2168-0450
Copyright note: Copyright © 2017 Simmons et al. Published by the Botanical Society of America. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC-BY-NC-SA 4.0), which permits unrestricted noncommercial use and redistribution provided that the original author and source are credited and the new work is distributed under the same license as the original.
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy; School of Science, Technology and Engineering; School of Law and Society; Centre for Bioinnovation
Language: English
Record Identifier: 99450699102621
Output Type: Journal article

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