Journal article
Characterization of Aplysia enticin and temptin, two novel water-borne protein pheromones that act in concert with attractin to stimulate mate attraction
Journal of Biological Chemistry, Vol.279(24), pp.25614-25622
2004
Abstract
Mate attraction in Aplysia involves a long-distance water-borne signal (attractin) that is released during egg laying. Other pheromones are predicted to be released during egg laying that act in concert with albumen gland attractin to stimulate attraction, but their identities are unknown. To identify other candidate water-borne pheromones, we employed differential library screening of an albumen gland cDNA library, Northern blot analysis, purification, characterization, cloning, and expression of albumen gland proteins, matrix-assisted laser desorption ionization mass spectrometry, pheromone secretion assays, behavioral bioassays, immunolocalization studies, and comparative genomics. Four genes, Alb-23, Alb-24, Alb-69, and Alb-172, were highly expressed in Aplysia californica albumen glands and encoded novel proteins. The products of the Alb-24 ("enticin") and Alb-172 ("temptin") precursors were soluble and highly abundant in albumen gland extracts, whereas Alb-23 and Alb-69 were membrane-associated proteins. A comparative analysis showed that the predicted Aplysia brasiliana enticin and temptin proteins were 90 and 91% identical, respectively, to their A. californica homologs. T-maze attraction bioassay studies have previously demonstrated that egg cordons alone are attractive to Aplysia but that attractin alone is not. In the present study, however, the combination of attractin, enticin, and temptin was found to be significantly attractive to potential mates and doubled the number of animals attracted to this stimulus compared with control animals. The combined data strongly suggest that enticin and temptin are novel candidate water-borne protein pheromones that act in concert with attractin to attract Aplysia to form and maintain egg-laying and mating aggregations.
Details
- Title
- Characterization of Aplysia enticin and temptin, two novel water-borne protein pheromones that act in concert with attractin to stimulate mate attraction
- Authors
- Scott F Cummins (Author) - University of Texas, United StatesA E Nichols (Author) - University of Texas, United StatesA Amare (Author) - University of Illinois, United StatesA B Hummon (Author) - University of Illinois, United StatesJ V Sweedler (Author) - University of Illinois, United StatesG T Nagle (Author) - University of Texas, United States
- Publication details
- Journal of Biological Chemistry, Vol.279(24), pp.25614-25622
- Publisher
- American Society for Biochemistry and Molecular Biology, Inc.
- Date published
- 2004
- DOI
- 10.1074/jbc.M313585200
- ISSN
- 0021-9258
- Copyright note
- Copyright © 2004 The American Society for Biochemistry and Molecular Biology. This research was originally published in Journal of Biological Chemistry. Author(s). Title. Journal of Biological Chemistry. 2004; 279:25614-25622. © the American Society for Biochemistry and Molecular Biology.
- Organisation Unit
- School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99450350702621
- Output Type
- Journal article
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- Collaboration types
- Domestic collaboration
- Web Of Science research areas
- Biochemistry & Molecular Biology