Characterization, expression, and function of the pyrokinins (PKs) in the giant freshwater prawn, Macrobrachium rosenbergii

Tipsuda Thongbuakaew; Sirirak  Mukem; Arada Chaiyamoon; Kanjana Khornchatri; Thanapong Kruangkum; Scott Cummins; Prasert Sobhon

doi:10.1242/jeb.243742

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Characterization, expression, and function of the pyrokinins (PKs) in the giant freshwater prawn, Macrobrachium rosenbergii

Journal article

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Characterization, expression, and function of the pyrokinins (PKs) in the giant freshwater prawn, Macrobrachium rosenbergii

Tipsuda Thongbuakaew, Sirirak Mukem, Arada Chaiyamoon, Kanjana Khornchatri, Thanapong Kruangkum, Scott Cummins and Prasert Sobhon

Journal of Experimental Biology, Vol.225(11), pp.1-10

2022

DOI: https://doi.org/10.1242/jeb.243742

PMID: 35578905

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Abstract

Macrobrachium rosenbergii

Giant freshwater prawn

Pyrokinin

Reproduction

Pyrokinins (PKs) are neuropeptides that have been found to regulate a variety of physiological activities including reproduction in various insect and crustacean species. However, the reproductive roles of PKs in the giant freshwater prawn have not yet been investigated. In this study, we identified the MroPK gene from next-generation sequence resources, which encodes a MroPK precursor that shares a high degree of conservation with the C-terminal sequence of FxPRLamide in other arthropods. MroPK is expressed within most tissues, except the hepatopancreas, stomach, and gill. Within developing ovarian tissue, MroPK expression was found to be significantly higher during the early stages (stages 1-2) compared with the late stages (stages 3-4), and could be localized to the oogonia, previtellogenic, and early vitellogenic oocytes. A role for PK in M. rosenbergii reproduction was supported following experimental administration of MroPK to ovarian explant cultures, showing an increase in the productions of progesterone and estradiol and upregulation of steroidogenesis-related genes (3β-HSD and 17β-HSD) and vitellogenin (Vg) expressions. Together, these results support a role for MroPK in regulating ovarian maturation via steroidogenesis.

Details

Title: Characterization, expression, and function of the pyrokinins (PKs) in the giant freshwater prawn, Macrobrachium rosenbergii
Authors: Tipsuda Thongbuakaew (Author) - Walailak University
Sirirak Mukem (Author) - Walailak University
Arada Chaiyamoon (Author) - Khon Kaen University
Kanjana Khornchatri (Author) - Thammasat University
Thanapong Kruangkum (Author) - Mahidol University
Scott Cummins (Author) - University of the Sunshine Coast, Queensland, GeneCology Research Centre - Legacy
Prasert Sobhon (Author) - Mahidol University
Publication details: Journal of Experimental Biology, Vol.225(11), pp.1-10
Publisher: The Company of Biologists Ltd.
Date published: 2022
DOI: 10.1242/jeb.243742
ISSN: 1477-9145
PMID: 35578905
Organisation Unit: University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99640578902621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Biology; Zoology

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