Characterization and multiplexing of EST-SSR primers in Cynodon (Poaceae) species

M C Jewell; Celine H Frere; P J Prentis; C J Lambrides; I D Godwin

doi:10.3732/ajb.1000254

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Characterization and multiplexing of EST-SSR primers in Cynodon (Poaceae) species

Journal article

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Characterization and multiplexing of EST-SSR primers in Cynodon (Poaceae) species

M C Jewell, Celine H Frere, P J Prentis, C J Lambrides and I D Godwin

American Journal of Botany, Vol.97(10), pp.e99-e101

2010

DOI: https://doi.org/10.3732/ajb.1000254

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Abstract

bermudagrass

diversity

grass

multiplex

polyploidy

SSR

Premise of the study: Cynodon species are multiple-use grasses that display varying levels of adaptation to biotic and abiotic stress. Previously identified EST-SSR primers were characterized and multiplexed to assess the level of genetic diversity present within a collection of almost 1200 Cynodon accessions from across Australia. Methods and Results: Two multiplex reactions were developed comprising a total of 16 EST-SSR markers. All SSR markers amplified across different Cynodon species and different levels of ploidy. The number of alleles ranged from one to eight per locus and the total number of alleles for the germplasm collection was 79. Conclusions: The 16 markers show sufficient variation for the characterization of Cynodon core collections and analysis of population genetic diversity in Cynodon grasses

Details

Title: Characterization and multiplexing of EST-SSR primers in Cynodon (Poaceae) species
Authors: M C Jewell (Author) - University of Queensland
Celine H Frere (Author) - University of Queensland
P J Prentis (Author) - University of Queensland
C J Lambrides (Author) - University of Queensland
I D Godwin (Author) - University of Queensland
Publication details: American Journal of Botany, Vol.97(10), pp.e99-e101
Publisher: Botanical Society of America, Inc.
Date published: 2010
DOI: 10.3732/ajb.1000254
ISSN: 0002-9122
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering
Language: English
Record Identifier: 99448960502621
Output Type: Journal article

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