Characterisation of enterovirus 71 replication kinetics in human colorectal cell line, HT29

Y L E Lui; Peter Timms; L M Hafner; T L Tan; K H Tan; E L Tan

doi:10.1186/2193-1801-2-267

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Characterisation of enterovirus 71 replication kinetics in human colorectal cell line, HT29

Journal article

Open access

Peer reviewed

Characterisation of enterovirus 71 replication kinetics in human colorectal cell line, HT29

Y L E Lui, Peter Timms, L M Hafner, T L Tan, K H Tan and E L Tan

SpringerPlus, Vol.2(267)

2013

DOI: https://doi.org/10.1186/2193-1801-2-267

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Abstract

colorectal cell

enterovirus 71

foot and mouth disease

hand

virus replication kinetics

Hand, Foot and Mouth Disease (HFMD), a contagious viral disease that commonly affects infants and children with blisters and flu like symptoms, is caused by a group of enteroviruses such as Enterovirus 71 (EV71) and coxsackievirus A16 (CA16). However some HFMD caused by EV71 may further develop into severe neurological complications such as encephalitis and meningitis. The route of transmission was postulated that the virus transmit from one person to another through direct contact of vesicular fluid or droplet from the infected or via faecal-oral route. To this end, this study utilised a human colorectal adenocarcinoma cell line (HT29) with epithelioid morphology as an in vitro model for the investigation of EV71 replication kinetics. Using qPCR, viral RNA was first detected in HT29 cells as early as 12 h post infection (hpi) while viral protein was first detected at 48 hpi. A significant change in HT29 cells' morphology was also observed after 48 hpi. Furthermore HT29 cell viability also significantly decreased at 72 hpi. Together, data from this study demonstrated that co-culture of HT29 with EV71 is a useful in vitro model to study the pathogenesis of EV71. © 2013 Lui et al.

Details

Title: Characterisation of enterovirus 71 replication kinetics in human colorectal cell line, HT29
Authors: Y L E Lui (Author) - Queensland University of Technology
Peter Timms (Author) - Queensland University of Technology
L M Hafner (Author) - Queensland University of Technology
T L Tan (Author) - Queensland University of Technology
K H Tan (Author) - Queensland University of Technology
E L Tan (Author) - Queensland University of Technology
Publication details: SpringerPlus, Vol.2(267); 8
Publisher: SpringerOpen
Date published: 2013
DOI: 10.1186/2193-1801-2-267
ISSN: 2193-1801
Copyright note: Copyright © 2013 Lui et al.; licensee Springer. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Organisation Unit: University of the Sunshine Coast, Queensland; Centre for Bioinnovation
Language: English
Record Identifier: 99448637602621
Output Type: Journal article

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Web Of Science research areas: Virology

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