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Characterisation of MHC class I genes in the koala
Journal article   Peer reviewed

Characterisation of MHC class I genes in the koala

Yuanyuan Cheng, Adam Polkinghorne, Amber Gillett, Elizabeth A Jones, Denis O'Meally, Peter Timms and Katherine Belov
Immunogenetics, Vol.70(2), pp.125-133
2018
url
https://doi.org/10.1007/s00251-017-1018-2View
Published Version

Abstract

Marsupial Koala MHC Class I
Koala (Phascolarctos cinereus) populations are on the decline across the majority of Australia's mainland. Two major diseases threatening the long-term survival of affected koala populations are caused by obligate intracellular pathogens: Chlamydia and koala retrovirus (KoRV). To improve our understanding of the koala immune system, we characterised their major histocompatibility complex (MHC) class I genes, which are centrally involved in presenting foreign peptides derived from intracellular pathogens to cytotoxic T cells. A total of 11 class I genes were identified in the koala genome. Three genes, Phci-UA, UB and UC, showed relatively high genetic variability and were expressed in all 12 examined tissues, whereas the other eight genes had tissue-specific expression and limited polymorphism. Evidence of diversifying selection was detected in Phci-UA and UC, while gene conversion may have played a role in creating new alleles at Phci-UB. We propose that Phci-UA, UB and UC are likely classical MHC genes of koalas, and further research is needed to understand their role in koala chlamydial and KoRV infections.

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Domestic collaboration
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Genetics & Heredity
Immunology

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