Changes in the neuropeptide content of Biomphalaria ganglia nervous system following Schistosoma infection

Tianfang Wang; Min Zhao; Di Liang; Utpal Bose; Satwant Kaur; Donald P McManus; Scott F Cummins

doi:10.1186/s13071-017-2218-1

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Changes in the neuropeptide content of Biomphalaria ganglia nervous system following Schistosoma infection

Journal article

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Changes in the neuropeptide content of Biomphalaria ganglia nervous system following Schistosoma infection

Tianfang Wang, Min Zhao, Di Liang, Utpal Bose, Satwant Kaur, Donald P McManus and Scott F Cummins

Parasites & vectors, Vol.10, 275

2017

DOI: https://doi.org/10.1186/s13071-017-2218-1

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Abstract

Biomphalaria

Schistosoma

neuropeptide

pre-patent infection

protein-protein interaction

proteomics

host-parasite interaction

Background: Molluscs, including snails, are prone to parasite infection, which can lead to massive physiological and behavioural changes, yet many of the molecular components involved remain unresolved. Central to this point is the neural system that in snails consists of several ganglia that regulate the animals' physiology and behaviour patterns. The availability of a genomic resource for the freshwater snail Biomphalaria glabrata provides a mean towards the high throughput analysis of changes in the central nervous system (CNS) following infection with Schistosoma miracidia. Results: In this study, we performed a proteomic analysis of the B. glabrata CNS at pre-patent infection, providing a list of proteins that were further used within a protein-protein interaction (PPI) framework against S. mansoni proteins. A hub with most connections for both non-infected and infected Biomphalaria includes leucine aminopeptidase 2 (LAP2), which interacts with numerous miracidia proteins that together belong to the immunoglobulin family of cell adhesion related molecules. We additionally reveal the presence of at least 165 neuropeptides derived from the precursors of buccalin, enterin, FMRF, FVRI, pedal peptide 1, 2, 3 and 4, RYamide, RFamide, pleurin and others. Many of these were present at significantly reduced levels in the snail's CNS post-infection, such as the egg laying hormone, a neuropeptide required to initiate egg laying in gastropod molluscs. Conclusions: Our analysis demonstrates that LAP2 may be a key component that regulates parasite infection physiology, as well as establishing that parasite-induced reproductive castration may be facilitated by significant reductions in reproduction-associated neuropeptides. This work helps in our understanding of molluscan neuropeptides and further stimulates advances in parasite-host interactions.

Details

Title: Changes in the neuropeptide content of Biomphalaria ganglia nervous system following Schistosoma infection
Authors: Tianfang Wang (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Min Zhao (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Di Liang (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Utpal Bose (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Satwant Kaur (Author) - Brunel University London, United Kingdom
Donald P McManus (Author) - QIMR Berghofer Medical Research Institute
Scott F Cummins (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Publication details: Parasites & vectors, Vol.10, 275; 13
Publisher: BioMed Central Ltd.
Date published: 2017
DOI: 10.1186/s13071-017-2218-1
ISSN: 1756-3305
Copyright note: Copyright © The Author(s). 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99451170002621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Parasitology; Tropical Medicine