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Published VersionCC BY V4.0, Open Access
Published VersionCC BY V4.0, Open
Journal article
Changes in Essential Fatty Acids and Ileal Genes Associated with Metabolizing Enzymes and Fatty Acid Transporters in Rodent Models of Cystic Fibrosis
International Journal of Molecular Sciences, Vol.24(8), pp.1-10
2023
PMID: 37108362
Abstract
Cystic fibrosis (CF), the result of mutations in the CF transmembrane conductance regulator (CFTR), causes essential fatty acid deficiency. The aim of this study was to characterize fatty acid handling in two rodent models of CF; one strain which harbors the loss of phenylalanine at position 508 (Phe508del) in CFTR and the other lacks functional CFTR (510X). Fatty acid concentrations were determined using gas chromatography in serum from Phe508del and 510X rats. The relative expression of genes responsible for fatty acid transport and metabolism were quantified using real-time PCR. Ileal tissue morphology was assessed histologically. There was an age-dependent decrease in eicosapentaenoic acid and the linoleic acid:α-linolenic acid ratio, a genotype-dependent decrease in docosapentaenoic acid (n-3) and an increase in the arachidonic acid:docosahexaenoic acid ratio in Phe508del rat serum, which was not observed in 510X rats. In the ileum, Cftr mRNA was increased in Phe508del rats but decreased in 510X rats. Further, Elvol2, Slc27a1, Slc27a2 and Got2 mRNA were increased in Phe508del rats only. As assessed by Sirius Red staining, collagen was increased in Phe508del and 510X ileum. Thus, CF rat models exhibit alterations in the concentration of circulating fatty acids, which may be due to altered transport and metabolism, in addition to fibrosis and microscopic structural changes in the ileum.
Details
- Title
- Changes in Essential Fatty Acids and Ileal Genes Associated with Metabolizing Enzymes and Fatty Acid Transporters in Rodent Models of Cystic Fibrosis
- Authors
- Nirajan Shrestha (Author) - Griffith UniversityNathan Rout-Pitt (Author) - The University of AdelaideAlexandra McCarron (Author) - The University of AdelaideCourtney A. Jackson (Author) - Griffith UniversityAndrew C. Bulmer (Author) - Griffith UniversityAndrew J. McAinch (Author) - Australian Institute for Musculoskeletal ScienceMartin Donnelley (Author) - The University of AdelaideDavid W. Parsons (Author) - The University of AdelaideDeanne H. Hryciw (Corresponding Author) - Griffith University
- Publication details
- International Journal of Molecular Sciences, Vol.24(8), pp.1-10
- Publisher
- MDPI AG
- Date published
- 2023
- DOI
- 10.3390/ijms24087194
- ISSN
- 1422-0067; 1661-6596
- PMID
- 37108362
- Copyright note
- © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).
- Grant note
- USA Cystic Fibrosis Foundation (PARSON18GO and DONNEL21GO).
- Organisation Unit
- School of Health - Biomedicine
- Language
- English
- Record Identifier
- 991186522002621
- Output Type
- Journal article
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