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Cellular stages of root formation, root system quality and survival of Pinuselliottii var. elliottii × P. caribaea var. hondurensis cuttings in different temperature environments
Journal article   Peer reviewed

Cellular stages of root formation, root system quality and survival of Pinuselliottii var. elliottii × P. caribaea var. hondurensis cuttings in different temperature environments

A Rasmussen, Tim E Smith and Mark A Hunt
New Forests, Vol.38(3), pp.285-294
2009
url
https://doi.org/10.1007/s11056-009-9147-6View
Published Version

Abstract

propagation stem anatomy root anatomy callus cuttings cortex abiotic Pinus
Time to first root in cuttings varies under different environmental conditions and understanding these differences is critical for optimizing propagation of commercial forestry species. Temperature environment (15, 25, 30 or 35 ± 2°C) had no effect on the cellular stages in root formation of the Slash × Caribbean Pine hybrid over 16 weeks as determined by histology. Initially callus cells formed in the cortex, then tracheids developed and formed primordia leading to external roots. However, speed of development followed a growth curve with the fastest development occurring at 25°C and slowest at 15°C with rooting percentages at week 12 of 80 and 0% respectively. Cutting survival was good in the three cooler temperature regimes (>80%) but reduced to 59% at 35°C. Root formation appeared to be dependant on the initiation of tracheids because all un-rooted cuttings had callus tissue but no tracheids, irrespective of temperature treatment and clone.

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