Journal article
Cardiac-specific overexpression of perilipin 5 provokes severe cardiac steatosis via the formation of a lipolytic barrier
Journal of Lipid Research, Vol.54(4), pp.1092-1102
2013
Abstract
Cardiac triacylglycerol (TG) catabolism critically depends on the TG hydrolytic activity of adipose triglyceride lipase (ATGL). Perilipin 5 (Plin5) is expressed in cardiac muscle (CM) and has been shown to interact with ATGL and its coactivator comparative gene identification-58 (CGI-58). Furthermore, ectopic Plin5 expression increases cellular TG content and Plin5-deficient mice exhibit reduced cardiac TG levels. In this study we show that mice with cardiac muscle-specific overexpression of perilipin 5 (CM-Plin5) massively accumulate TG in CM, which is accompanied by moderately reduced fatty acid (FA) oxidizing gene expression levels. Cardiac lipid droplet (LD) preparations from CM of CM-Plin5 mice showed reduced ATGLand hormone-sensitive lipase-mediated TG mobilization implying that Plin5 overexpression restricts cardiac lipolysis via the formation of a lipolytic barrier. To test this hypothesis, we analyzed TG hydrolytic activities in preparations of Plin5-, ATGL-, and CGI-58-transfected cells. In vitro ATGLmediated TG hydrolysis of an artificial micellar TG substrate was not inhibited by the presence of Plin5, whereas Plin5-coated LDs were resistant toward ATGL-mediated TG catabolism. These findings strongly suggest that Plin5 functions as a lipolytic barrier to protect the cardiac TG pool from uncontrolled TG mobilization and the excessive release of free FAs. Copyright © 2013 by the American Society for Biochemistry and Molecular Biology, Inc.
Details
- Title
- Cardiac-specific overexpression of perilipin 5 provokes severe cardiac steatosis via the formation of a lipolytic barrier
- Authors
- Nina M Pollak (Author) - University of Graz, AustriaM Schweiger (Author) - University of Graz, AustriaD Jaeger (Author) - University of Graz, AustriaD Kolb (Author) - Medical University of Graz, AustriaM Kumari (Author) - University of Graz, AustriaR Schreiber (Author) - University of Graz, AustriaS Kolleritsch (Author) - University of Graz, AustriaP Markolin (Author) - University of Graz, AustriaG F Grabner (Author) - University of Graz, AustriaC Heier (Author) - University of Graz, AustriaK A Zierler (Author) - University of Graz, AustriaT Rülicke (Author) - University of Veterinary Medicine, AustriaR Zimmermann (Author) - University of Graz, AustriaA Lass (Author) - University of Graz, AustriaR Zechner (Author) - University of Graz, AustriaG Haemmerle (Author) - University of Graz, Austria
- Publication details
- Journal of Lipid Research, Vol.54(4), pp.1092-1102
- Publisher
- American Society for Biochemistry and Molecular Biology, Inc.
- Date published
- 2013
- DOI
- 10.1194/jlr.M034710
- ISSN
- 0022-2275
- Copyright note
- Copyright © 2013 by the American Society for Biochemistry and Molecular Biology, Inc. The published version is reproduced here in accordance with the publisher's copyright policy
- Organisation Unit
- School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99451026602621
- Output Type
- Journal article
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