Capillary ultrastructure and mitochondrial volume density in skeletal muscle in relation to reduced exercise capacity of patients with intermittent claudication

Oliver Baum; Eleonora Torchetti; Corinna Malik; Birgitte Hoier; Meegan A Walker; Philip Walker; Adolfo Odriozola; Franziska Graber; Stefan Tschanz; Jens Bangsbo; Hans Hoppeler; Christopher D Askew; Ylva Hellsten

doi:10.1152/ajpregu.00480.2015

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Capillary ultrastructure and mitochondrial volume density in skeletal muscle in relation to reduced exercise capacity of patients with intermittent claudication

Journal article

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Capillary ultrastructure and mitochondrial volume density in skeletal muscle in relation to reduced exercise capacity of patients with intermittent claudication

Oliver Baum, Eleonora Torchetti, Corinna Malik, Birgitte Hoier, Meegan A Walker, Philip Walker, Adolfo Odriozola, Franziska Graber, Stefan Tschanz, Jens Bangsbo, …

American Journal of Physiology: Regulatory Integrative and Comparative Physiology, Vol.310(10), pp.R943-R951

2016

DOI: https://doi.org/10.1152/ajpregu.00480.2015

PMID: 27009051

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Abstract

skeletal muscle

transmission electron microscopy

capillary

morphometry

peripheral arterial disease

Intermittent claudication (IC) is the most commonly reported symptom of peripheral arterial disease (PAD). Impaired limb blood flow is a major casual factor of lower exercise tolerance in PAD, but cannot entirely explain it. We hypothesized that IC is associated with structural changes of the capillary-mitochondria interface that could contribute to the reduction of exercise tolerance in IC-patients. Capillary and mitochondrial morphometry were performed after light and transmission electron microscopy using vastus lateralis muscle biopsies of 14 IC-patients and 10 age-matched controls and peak power output (PPO) was determined for all participants using an incremental single-leg knee-extension protocol. Capillary density was lower (411±90 mm-2 versus 506±95 mm-2; P≤0.05) in the biopsies of the IC patients than in those of the controls. The basement membrane (BM) around capillaries was thicker (543±82 nm versus 423±97 nm; P≤0.01) and the volume density of mitochondria was lower (3.51±0.56% versus 4.60±0.74; P≤0.01) in the IC-patients than the controls. In the IC-patients, a higher proportion of capillaries appeared with collapsed slit-like lumen and/or swollen endothelium. PPO was lower (18.5±9.9 W versus 33.5±9.4 W; P≤0.01) in the IC-patients than the controls. We suggest that several structural alterations in skeletal muscle, either collectively or separately, contribute to the reduction of exercise tolerance in IC-patients.

Details

Title: Capillary ultrastructure and mitochondrial volume density in skeletal muscle in relation to reduced exercise capacity of patients with intermittent claudication
Authors: Oliver Baum (Author) - University of Bern, Switzerland
Eleonora Torchetti (Author) - University of Bern, Switzerland
Corinna Malik (Author) - University of Bern, Switzerland
Birgitte Hoier (Author) - University of Copenhagen, Denmark
Meegan A Walker (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Philip Walker (Author) - University of Queensland
Adolfo Odriozola (Author) - University of Bern, Switzerland
Franziska Graber (Author) - University of Bern, Switzerland
Stefan Tschanz (Author) - University of Bern, Switzerland
Jens Bangsbo (Author) - University of Copenhagen, Denmark
Hans Hoppeler (Author) - University of Bern, Switzerland
Christopher D Askew (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Ylva Hellsten (Author) - University of Copenhagen, Denmark
Publication details: American Journal of Physiology: Regulatory Integrative and Comparative Physiology, Vol.310(10), pp.R943-R951
Publisher: American Physiological Society
Date published: 2016
DOI: 10.1152/ajpregu.00480.2015
ISSN: 0363-6119
PMID: 27009051
Copyright note: Copyright © 2016 American Physiological Society. The author's accepted version is reproduced here in accordance with the publisher's copyright policy. The final definitive version is available at: http://dx.doi.org/10.1152/ajpregu.00480.2015
Grants: National Centre of Research Excellence to Improve Management of Peripheral Arterial Disease, 1000967, National Health and Medical Research Council (Australia, Canberra) - NHMRC
Grant note: This study was financially supported by a grant from the Swiss National Science Foundation (SNF, : 320030-144167), a Seed Research Grant from the University of the Sunshine Coast, Australia (, SRG 09-08), and The Danish Council for Independent Research-Medical Sciences.
Organisation Unit: School of Health and Sport Sciences - Legacy; School of Health - Sports & Exercise Science; School of Health and Behavioural Sciences - Legacy
Language: English
Record Identifier: 99449914602621
Output Type: Journal article

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