Can germ cell neoplasia in situ be diagnosed by measuring serum levels of microRNA371a-3p?

A Radtke; J-F Cremers; S Kliesch; Stephan Riek; K Junker; S A Mohamed; P Anheuser; G Belge; K-P Dieckmann

doi:10.1007/s00432-017-2490-7

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Can germ cell neoplasia in situ be diagnosed by measuring serum levels of microRNA371a-3p?

Journal article

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Can germ cell neoplasia in situ be diagnosed by measuring serum levels of microRNA371a-3p?

A Radtke, J-F Cremers, S Kliesch, Stephan Riek, K Junker, S A Mohamed, P Anheuser, G Belge and K-P Dieckmann

Journal of Cancer Research and Clinical Oncology, Vol.143(11), pp.2383-2392

2017

DOI: https://doi.org/10.1007/s00432-017-2490-7

PMCID: PMC5640733

PMID: 28819887

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Abstract

Testicular Neoplasms - diagnosis

Testicular Neoplasms - genetics

Prognosis

Follow-Up Studies

Neoplasm Invasiveness

Humans

Middle Aged

Male

Survival Rate

Neoplasms, Germ Cell and Embryonal - genetics

Neoplasms, Germ Cell and Embryonal - blood

Case-Control Studies

Neoplasms, Germ Cell and Embryonal - diagnosis

Biomarkers, Tumor - blood

Carcinoma in Situ - genetics

Adolescent

Adult

Biomarkers, Tumor - genetics

MicroRNAs - blood

MicroRNAs - genetics

Carcinoma in Situ - diagnosis

Neoplasm Staging

Testicular Neoplasms - blood

Carcinoma in Situ - blood

Diagnosing germ cell neoplasia in situ (GCNis) can detect germ cell tumours (GCTs) at the pre-invasive stage. To date, testicular biopsy with the potential of surgical complications is the only way of safely diagnosing GCNis. Recently, microRNAs (miRs) 371-3, and miR 367 were shown to be valuable serum biomarkers of GCTs. We explored the usefulness of these candidate miRs as a marker for GCNis. 27 patients with GCNis and no concomitant GCT were enrolled. All patients underwent measuring serum levels of miR-371a-3p and miR-367-3p before treatment, 11 had repeat measurement after treatment, 2 also had testicular vein blood examinations. Serum levels were measured by quantitative PCR. In addition, four orchiectomy specimens of patients with GCT were examined immunohistochemically and by in situ hybridization (ISH) with a probe specific for miR-371a-3p to look for the presence of this miR in GCNis cells. The median serum level of miR-371a-3p was significantly higher in patients with GCNis than in controls, miR-367 levels were not elevated. Overall, 14 patients (51.9%) had elevated serum levels of miR-371a-3p. The highest levels were found in patients with bilateral GCNis. Levels in testicular vein serum were elevated in both of the cases. After treatment, all elevated levels dropped to normal. In two orchiectomy specimens, miR-371a-3p was detected by ISH in GCNis cells. Measuring miR-371a-3p serum levels can replace control biopsies after treatment of GCNis. In addition, the test can guide clinical decision making regarding the need of testicular biopsy in cases suspicious of GCNis.

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Title: Can germ cell neoplasia in situ be diagnosed by measuring serum levels of microRNA371a-3p?
Authors: A Radtke (Author) - University of Bremen
J-F Cremers (Author) - University of Muenster
S Kliesch (Author) - University of Muenster
Stephan Riek (Author) - University of Bremen
K Junker (Author) - Klinikum Bremen-Mitte
S A Mohamed (Author) - University of Luebeck
P Anheuser (Author) - Albertinen Krankenhaus
G Belge (Author) - University of Bremen
K-P Dieckmann (Author) - Albertinen Krankenhaus
Publication details: Journal of Cancer Research and Clinical Oncology, Vol.143(11), pp.2383-2392
Publisher: Springer
Date published: 2017
DOI: 10.1007/s00432-017-2490-7
ISSN: 1432-1335; 0171-5216
PMID: 28819887; PMC5640733
Copyright note: This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Organisation Unit: Graduate Research School; University of the Sunshine Coast, Queensland
Language: English
Record Identifier: 99472607102621
Output Type: Journal article

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