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Callus culture as a new approach for the production of high added value compounds in Ilex paraguariensis - genotype influence medium optimization and compounds identification2.34 MB
Published VersionCC BY V4.0, Open Access
Journal article
Callus culture as a new approach for the production of high added value compounds in Ilex paraguariensis: genotype influence, medium optimization and compounds identification
Anais da Academia Brasileira de Ciências, Vol.92(3), pp.1-16
2020
Abstract
Ilex paraguariensis (yerba mate) is a native species from South America and is a rich source of bioactive compounds. There is a lack of research efforts on the phytochemical investigation of callus culture from this species. In the present study, an effort was made to optimize callus culture conditions and to identify secondary compounds. Calli were induced from 10 genotypes using leaf explants and the best genotype was selected to evaluate the effects of cytokinin types and concentrations on callus induction and biomass accumulation. The best genotype and cytokinin treatment were used to conduct one last experiment with sucrose concentrations in culture media and its effects on calli biomass, antioxidant activity and secondary compounds accumulation. Callus initiation was genotype dependent, and the 6-156-6 line had the best response. Zeatin supplemented medium showed higher callus induction rate (82%) and higher biomass accumulation after 120 days (328.2 mg). Higher biomass and secondary compounds accumulation were observed for calli on 3% sucrose medium. Antioxidant activity was not affected by sucrose concentrations. Yerba mate callus culture allowed the accumulation of chlorogenic acid, cryptochlorogenic acid, neochlorogenic acid, 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid, theobromine and caffeine.
Details
- Title
- Callus culture as a new approach for the production of high added value compounds in Ilex paraguariensis: genotype influence, medium optimization and compounds identification
- Authors
- Renata Grunennvaldt (Author) - University of the Sunshine Coast, Queensland, School of Science and Engineering - LegacyJuliana Degenhardt-Goldbach (Author) - National Centre of Forestry ResearchPeter Brooks (Author) - University of the Sunshine Coast, Queensland, School of Science and Engineering - LegacyJessica De Cassia Tomasi (Author) - Universidade Federal do ParanáFabricio Augusto Hansel (Author) - National Centre of Forestry ResearchTrong Tran (Author) - University of the Sunshine Coast, Queensland, School of Science and Engineering - LegacyErik N Gomes (Author) - Rutgers, The State University of New JerseyCicero Deschamps (Author) - Universidade Federal do Paraná
- Publication details
- Anais da Academia Brasileira de Ciências, Vol.92(3), pp.1-16
- Publisher
- Academia Brasileira de Ciencias
- Date published
- 2020
- DOI
- 10.1590/0001-3765202020181251
- ISSN
- 1678-2690; 0001-3765
- Copyright note
- This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) 4.0 License.
- Organisation Unit
- School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99489708402621
- Output Type
- Journal article
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