Journal article
Calcium determines the supramolecular organization of fibrillin-rich microfibrils
Journal of Cell Biology, Vol.141(3), pp.829-837
1998
Abstract
Microfibrils are ubiquitous fibrillin-rich polymers that are thought to provide long-range elasticity to extracellular matrices, including the zonular filaments of mammalian eyes. X-ray diffraction of hydrated bovine zonular filaments demonstrated meridional diffraction peaks indexing on a fundamental axial periodicity (D) of approximately 56 nm. A Ca2+-induced reversible change in the intensities of the meridional Bragg peaks indicated that supramolecular rearrangements occurred in response to altered concentrations of free Ca2+. In the presence of Ca2+, the dominant diffracting subspecies were microfibrils aligned in an axial 0.33-D stagger. The removal of Ca2+ caused an enhanced regularity in molecular spacing of individual microfibrils, and the contribution from microfibrils not involved in staggered arrays became more dominant. Scanning transmission electron microscopy of isolated microfibrils revealed that Ca2+ removal or addition caused significant, reversible changes in microfibril mass distribution and periodicity. These results were consistent with evidence from x-ray diffraction. Simulated meridional x-ray diffraction profiles and analyses of isolated Ca2+-containing, staggered microfibrillar arrays were used to interpret the effects of Ca2+. These observations highlight the importance of Ca2+ to microfibrils and microfibrillar arrays in vivo.
Details
- Title
- Calcium determines the supramolecular organization of fibrillin-rich microfibrils
- Authors
- Tim J Wess (Author) - University of Stirling, United KingdomP P Purslow (Author) - Royal Veterinary and Agricultural University, DenmarkM J Sherratt (Author) - University of Manchester, United KingdomJ Ashworth (Author) - University of Manchester, United KingdomC A Shuttleworth (Author) - University of Manchester, United KingdomC M Kielty (Author) - University of Manchester, United Kingdom
- Publication details
- Journal of Cell Biology, Vol.141(3), pp.829-837
- Publisher
- Rockefeller University Press
- Date published
- 1998
- DOI
- 10.1083/jcb.141.3.829
- ISSN
- 0021-9525
- Organisation Unit
- Office of the Deputy Vice-Chancellor (Academic); University of the Sunshine Coast, Queensland
- Language
- English
- Record Identifier
- 99450860702621
- Output Type
- Journal article
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